HUMAnN 3.8 - How to get taxonomic results at the order level instead of genus?

[Jeremy_Tournayre]

Hello,

I found solution here:

Request for Higher Taxonomic Level Analysis in HUMAnN

Dear HUMAnN Development Team, I am currently utilizing the HUMAnN software for metagenomic analysis, specifically interested in taxonomic characterization at various levels of resolution. The output I am receiving is at the species level by default, and I have successfully used the humann_genefamilies_genus_level command to obtain results at the genus level. However, my research requires analysis at higher taxonomic levels beyond genus. Could you please advise if there is a way to modify the o…

with humann_infer_taxonomy

humann_infer_taxonomy -l Genus -d uniref90-tol-lca -i humann_genefamilies.tsv -o Genus/humann_genefamilies.tsv"

It works but I don’t know why there are some stange things like this below.
Why do I have “unclassified” instead of “g__Prevotella” and “g__Prevotellaceae_unclassified” in the Genus file?

Original “humann_genefamilies.tsv” :

UniRef90_A0A010YBU9 43.4636486692
UniRef90_A0A010YBU9|g__Prevotella.s__Prevotella_sp_ne3005 15.4012377598
UniRef90_A0A010YBU9|g__Prevotella.s__Prevotella_ruminicola 12.1212121212
UniRef90_A0A010YBU9|g__Prevotellaceae_unclassified.s__Prevotellaceae_bacterium_MN60 6.0506050605
UniRef90_A0A010YBU9|g__Prevotella.s__Prevotella_sp_tc2_28 4.4004400440
UniRef90_A0A010YBU9|g__Prevotella.s__Prevotella_sp_tf2_5 3.2789872934
UniRef90_A0A010YBU9|g__Prevotella.s__Prevotella_aff_ruminicola_Tc2_24 2.2111663903

In Phylum:

UniRef90_A0A010YBU9 43.4636
UniRef90_A0A010YBU9|p__Bacteroidetes 43.4636

In Genus:

UniRef90_A0A010YBU9 43.4636
UniRef90_A0A010YBU9 unclassified  43.4636

Best regards,
Jérémy Tournayre