Hi, I have a question about data transformation prior to input into Maaslin2.
I am thinking of transforming a metagenomics abundance dataset using centered log ratio, then input into Maaslin2 with parameters
transform = "none", normalization ="None". Other parameters remain default.
Is this allowed?
Reason for doing so is because I wanted to use Maaslin2’s capability to control for covariates but I could not find any other tool that is compatible with both covariate adjustment and centered log ratio transformation.