I’m using MaAsLin2 version 1.8.0
I have a longitudinal microbiome data and phenotype data (Presence/absence) of 8 visits. Instead of looking at the association between the phenotype and microbiome features for the entire 8 visits altogether, I want to explore whether there’s a consistent pattern where the microbiome feature in a preceding visit is associated with a phenotype (Presence/absence) observed in the next visit. It is more like a sequential relationship between microbiome features and phenotype across visits. The analysis aims to uncover patterns of microbiome dynamics that relate to changes in phenotype.