Hi @franzosa. I was reading the HUMAnN2 article (Franzosa et al. 2018, Nature Methods) and could not understand a particular concept. Can you please help me?
Here you have written:
“The tiered search generates mappings of meta’omic reads to gene
sequences with known or ambiguous taxonomy”
In the third tier, you do a translated search from the unmapped reads. And, to my knowledge, it gives you the protein that is encoded by that particular short read. I am not understanding how do you get the gene for that translated protein? Also, how do you get the length of the gene from that translated protein?