Hello, I am trying to interpreet these scatter plots that I have generated. These are specific taxa associated with ultraprocessed food consumption. Can anyone help me to understand it? why there are some sort of clusters?
Thanks for the question! Can you give us some more details? In particular, it would be good to know which transformation and normalization methods you are using.
Best,
Kelsey
Hi @Kelsey_Thompson I am not doing any transformation, just CLR normalization of counts.
Cheers
Alex
Hi Alex,
My intuition here is that this is a property of the underlying data. When MaAsLin plots the data it is the raw data that was supplied - not what the model is seeing (e.g. before MaAsLin may normalize or transform the data - depending on your settings). I’m happy to dig further if you can provide a minimally reproducible example - but I believe the two clusters are just for high abundant and low abundant samples of these microbes.
I hope this helps!
Best,
Kelsey