Yes, the method is practically identical, we introduced a couple of QC on the alignment quality and a new expanded database comprising 12k species (see https://forum.biobakery.org/t/can-you-tell-us-about-the-db-updates/310). We try to use maximum 150 markers per species, for complete marker statistics you can check the marker info file here https://www.dropbox.com/sh/7qze7m7g9fe2xjg/AAAlyQITZuUCtBUJxpxhIroIa/mpa_v30_CHOCOPhlAn_201901_marker_info.txt.bz2?dl=1