NOT getting to run MetaPhlAn

Microbial community profiling MetaPhlAn
1

Hi,
I am trying to run MetaPhlAn 3.0, but I am not getting to run it. It is showing the following error. I created conda environment and installed MetaPhlAn using pip, downloaded the database (metaphlan --install --bowtie2db bowtie2db/) and add in my environment PATH.
run command:

metaphlan assembly.fasta --input_type fasta > M25.txt

Thanks!

–error
No MetaPhlAn BowTie2 database found (–index option)!
Expecting location bowtie2db

2

Hi @sekhwal you should specify the parameter --bowtie2db bowtie2db/ as you installed the database in a custom directory