No species identified and empty pathway files created

Hello,

I received this message
humann v3.7
MetaPhlAn version 3.0.9 (17 May 2021)
Bowtie2 version 2.2.3

home/kbrokordt/miniconda3/lib/python3.12/site-packages/humann/quantify/MinPath12hmp.py:804: SyntaxWarning: invalid escape sequence ‘\d’
m = re.match(‘[1]+(?P\d+)’, aline)
/home/kbrokordt/miniconda3/lib/python3.12/site-packages/humann/quantify/MinPath12hmp.py:804: SyntaxWarning: invalid escape sequence ‘\d’
m = re.match(‘[2]+(?P\d+)’, aline)
Output files will be written to: /home/kbrokordt/camaron/Pruebas/nonrRNA/OUTOUT

Running metaphlan …

Total species selected from prescreen: 0

Selected species explain 0.00% of predicted community composition

No species were selected from the prescreen.
Because of this the custom ChocoPhlAn database is empty.
This will result in zero species-specific gene families and pathways.

Aligning to reference database: uniref90_201901b_full.dmnd

Total bugs after translated alignment: 1
unclassified: 5557552 hits

Total gene families after translated alignment: 14628

Unaligned reads after translated alignment: 97.5684571395 %

Any suggestion


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  2. ^\d ↩︎