Hi all (@chwang.mt, @Shailes_Bhattarai, @KL2003),
First, we apologize for this issue, we are aware that there is currently no access to our Galaxy site (or lab website) and are actively working on getting it back up. However, unfortunately, we do not have an ETA on when it will be accessible again and we apologize for the inconvenience.
In the meantime there are a couple of other options for doing this type of analysis, LEfSe itself has not been under development for quite some time in the lab.
- MaAsLin2 is our newer and still under maintenance tool to conduct DA analysis, it is more robust to the data and usually results in far fewer false positive hits from the model, see this manuscript for more information on DA methods or the MaAsLin manuscript for more details about the tool. Additionally, it allows for the incorporation of longitudinal data and allows for many covariates to be adjusted for. Here is a link to the tutorial for that tool.
- You can always install and run LEfSe on the command line.
I hope this helps in your analysis needs and again we apologize for this inconvenience.
Best,
Kelsey