Lefse without any subclass- still valid?

[darmecian]

LefSe: what happens if no subclass

Hello, I’ve seen this question asked a few times on the previous lefse forum but never answered succinctly. If one does not provide a subclass, does LefSe run the wilcoxon? If so, what is it testing and will the result differ from only the KW? If one does provide a subclass, will the results from the wilcoxon show up in the “.res” file? Thanks in advance! John

I think you have an answer here.