Hi there, I recently ran the maaslin2 analysis shown as below:
fit_data = Maaslin2(
input_data = abd,
input_metadata = meta,
min_prevalence = 0,
normalization = “NONE”,
transform = “NONE”,
output = “data/output_Maaslin2”,
fixed_effects = c(“Age_group”,“Defecating_Difficulty”), #two groups only
random_effects = “Sequencing_Batch”,
analysis_method = “CPLM”)
And I got some warnning message:
In fitTMB(TMBStruc) :
Model convergence problem; false convergence (8). See vignette(‘troubleshooting’)
How can I optimize my formula and do you have any idea or comments?
Thanks in advance,
YZ