Running melonnpan with a single sample

Can melonnpan predict be used with one sample only? If so, how is it possible?
It works fine for 3 samples or above, but I get trait is monomorphic error when I use a single sample, or trait is binary error if I use 2 samples (the input files are the merged output of the humann2 gene_families.tsv files normalized by “relab” and transposed), even if I set the no.transform.metag to TRUE, it is still problematic

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I have the same issue @franzosa