Minimum sample size for MelonnPan

Hello community,
I have a question about melonnpan.

I want to run the melonnpan with mouse gut microbiome data.
I have only 22 samples, however I realized that melonnpan is based on ML for predicting the metabolites.

Therefore, I wonder the appropriate sample size for the MelonnPan training.

Can I use 22 samples for the training?


Hi @MinJi_Kim - since you have a small sample size, you can use Leave One Out Cross Validation (LOOCV) by setting nfolds = 22 for MelonnPan training.

All the best,

I really appreciate it.
I’ll try it according to your recommendation.

Best wishes,