Dear Sir,
My name is Aditi Jangid, research scholar from India. I am working on metagenomic data. My aim is to explore the change in antibody expression with fecal microbiome community (longitudinal study). For the same I have prepared the metadata for MaAsLin2 and put antibody expression data on the metadata sheet as it is viz, absolute value (although my metagenomic data is normalized). Now, I need to know whether this is the correct method or do I need to normalize the antibody data first (similar way of normalization as microbiome data). For your reference find the metadata sheet attached. I request you to please provide your valuable suggestions. Your help is highly appreciated.
Thanks & regards
Aditi Jangid
Research Scholar
BioX Centre, School of Basic Sciences
Indian Institute of Technology, Mandi, India