How to get microbial relative contributors for PICRUSt2 result

Microbial community profiling PICRUSt
1

I want to know, Is there any way to get microbial contributors for the PICRUSt2 result? After generating pathway abundance, can I trace it back to investigate microbial contributors for each pathway? Is there a command line to do that? One solution that I know, we can calculate relative contributions as the total number of copies of genes mapped to a pathway across all samples per genus over the total number of gene copies assigned to that pathway.

I am grateful for your help.

2

Hi @bowornpol ,

Sorry for the late response, but you can indeed generate stratified picrust2 profiles to see what microbes are contributing to each predicted pathway. You can check out the tutorial on how to do this here:

using the --per_sequence_contrib flag

Cheers,
Jacob