Hi,
I just began to use this software. So in this post, sma says the normalization is done internally to get relative abundance. Does that mean if I don’t set -o parameter, the program would do it still automatically or do I have to set it? Additional question, in the tutorial, the -o set to 1000000, in the galaxy, kinda explained it a bit (Per-sample normalization of the sum of the values to 1M), but I am doing amplicon research, I am not sure what does that actually do to normalize your sample?
Thank you!