# Which folder should I put phy\_database in? **URL:** \ **Category:** PhyloPhlAn\ **Created:** [May 12, 2020, 5:08am UTC](https://forum.biobakery.org/t/which-folder-should-i-put-phy-database-in/464 "2020-05-12T05:08:53Z")\ **Posts on this page:** 2\ **Page:** 1
**Author:** ![CK\_zhu](https://yyz1.discourse-cdn.com/flex027/user_avatar/forum.biobakery.org/ck_zhu/32/151_2.png) [@CK\_zhu](https://forum.biobakery.org/u/CK_zhu)\ **Post date:** [May 12, 2020, 5:08am UTC](https://forum.biobakery.org/t/which-folder-should-i-put-phy-database-in/464/1 "2020-05-12T05:08:53Z")
Hi, I’ve been working on your tutorial recently ```auto https://github.com/biobakery/MetaPhlAn/wiki/StrainPhlAn-3.0 ``` # StrainPhlAn: metagenomic strain-level population genomics when I run step5, it says StrainPhlAn will call PhyloPhlAn to produce a multiple sequence alignment (MSA) to then build the phylogenetic tree. then I get an error ```auto strainphlan -s consensus_markers/*.pkl -m db_markers/s __Bacteroides_caccae.fna -r reference_genomes/G000273725.fna.bz2 -o output -n 8 -c s__ Bacteroides_caccae --phylophlan_mode accurate --mutation_rates Tue May 12 12:37:58 2020: Start StrainPhlAn 3.0 execution Tue May 12 12:37:58 2020: Creating temporary directory... Tue May 12 12:37:58 2020: Done. Tue May 12 12:37:58 2020: Getting markers from main sample files... Tue May 12 12:38:00 2020: Done. Tue May 12 12:38:00 2020: Getting markers from main reference files...Warning: [blastn] Examining 5 or more matches is recommended Tue May 12 12:38:17 2020: Done. Tue May 12 12:38:17 2020: Removing bad markers / samples... Tue May 12 12:38:17 2020: Done. Tue May 12 12:38:17 2020: Writing samples as markers' FASTA files... Tue May 12 12:38:18 2020: Done. Tue May 12 12:38:18 2020: Writing filtered clade markers as FASTA file... Tue May 12 12:38:18 2020: Done. Tue May 12 12:38:18 2020: Calculating polymorphic rates... Tue May 12 12:38:19 2020: Done. Tue May 12 12:38:19 2020: Executing PhyloPhlAn 3.0... Tue May 12 12:38:19 2020: Creating PhyloPhlAn 3.0 database... Tue May 12 12:38:23 2020: Done. Tue May 12 12:38:23 2020: Generating PhyloPhlAn 3.0 configuration file... Tue May 12 12:38:24 2020: Done. Tue May 12 12:38:24 2020: Processing samples...[e] unable to download "https://www.dropbox.com/s/x7cvma5bjzlllbt/phylophlan_databases.txt?dl=1" [e] An error was ocurred executing a external tool, exiting... Tue May 12 12:38:56 2020: Stop StrainPhlAn 3.0 execution. ``` I can’t download databases from wget, then I download this database from chrome. So my question is _ **Which folder should I put this database in?** _ my metaphlan3 version is `MetaPhlAn version 3.0 (20 Mar 2020)` ---
**Author:** ![f.asnicar](https://yyz1.discourse-cdn.com/flex027/user_avatar/forum.biobakery.org/f.asnicar/32/196_2.png) [@f.asnicar](https://forum.biobakery.org/u/f.asnicar)\ **Post date:** [May 12, 2020, 7:33am UTC](https://forum.biobakery.org/t/which-folder-should-i-put-phy-database-in/464/2 "2020-05-12T07:33:44Z")
Hi, thanks for reporting this. I think this should be under the StrainPhlAn category and most likely the best person to ask bout this is @aitor.blancomiguez who is developing StrainPhlAn. Many thanks, Francesco