# Unknown bowtie2 errors **URL:** \ **Category:** KneadData\ **Created:** [March 30, 2021, 9:58pm UTC](https://forum.biobakery.org/t/unknown-bowtie2-errors/1871 "2021-03-30T21:58:04Z")\ **Posts on this page:** 2\ **Page:** 1
**Author:** ![kochja](https://avatars.discourse-cdn.com/v4/letter/k/dfb087/32.png) [@kochja](https://forum.biobakery.org/u/kochja)\ **Post date:** [March 30, 2021, 9:58pm UTC](https://forum.biobakery.org/t/unknown-bowtie2-errors/1871/1 "2021-03-30T21:58:04Z")
I have tried kneaddata 0.6.1 and 0.7.4 with no luck running bowtie2. If I try running bowtie2 on its own I get: perl: symbol lookup error: /usr/lib64/perl5/auto/Fcntl/Fcntl.so: undefined symbol: Perl\_xs\_apiversion\_bootcheck If I try running kneaddata, the bowtie2 step gives: Error message returned from bowtie2 : /dfs/IB/Weis\_Lab/kochja/opt/conda/envs/kneaddata/bin/bowtie2-align-s: symbol lookup error: /dfs/IB/Weis\_Lab/kochja/opt/conda/envs/kneaddata/bin/bowtie2-align-s: undefined symbol: \_ZN3tbb10interface78internal15task\_arena\_base19internal\_initializeEv (ERR): Description of arguments failed! I have tried installing different versions of tbb based on this post: [Conda version does not work with most recent tbb version · Issue #336 · BenLangmead/bowtie2 · GitHub](https://github.com/BenLangmead/bowtie2/issues/336) I have tried installing older versions of bowtie2 as well. Thoughts? Thanks! ---
**Author:** ![cjburgess](https://yyz1.discourse-cdn.com/flex027/user_avatar/forum.biobakery.org/cjburgess/32/857_2.png) [@cjburgess](https://forum.biobakery.org/u/cjburgess)\ **Post date:** [June 15, 2021, 9:13pm UTC](https://forum.biobakery.org/t/unknown-bowtie2-errors/1871/2 "2021-06-15T21:13:33Z")
I dont’ know if you were able to fix this issue but I think I stumbled upon an answer [HERE](https://github.com/bioconda/bioconda-recipes/issues/4390#issuecomment-302396698). PERL5LIB=""; humann -i demo.fastq -o demo\_out