# Trouble running Humann 3.5 after update from Humann3

**URL:** <https://forum.biobakery.org/t/trouble-running-humann-3-5-after-update-from-humann3/4331>\
**Category:** HUMAnN\
**Created:** [November 2, 2022, 7:11am UTC](https://forum.biobakery.org/t/trouble-running-humann-3-5-after-update-from-humann3/4331 "2022-11-02T07:11:39Z")\
**Posts on this page:** 1\
**Page:** 1

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**Author:** ![Nadav\_Moriel](https://yyz1.discourse-cdn.com/flex027/user_avatar/forum.biobakery.org/nadav_moriel/32/1573_2.png) [@Nadav\_Moriel](https://forum.biobakery.org/u/Nadav_Moriel)\
**Post date:** [November 2, 2022, 7:11am UTC](https://forum.biobakery.org/t/trouble-running-humann-3-5-after-update-from-humann3/4331/1 "2022-11-02T07:11:40Z")

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Hi there,

We have recently updated our Humann version to 3.5 on our conda environment in our cluster. Unfortunately, although it works smoothly for the person that performed the installation, I keep on getting the following error message:

 ![image](https://canada1.discourse-cdn.com/flex027/uploads/biobakery/original/2X/d/df6e00642825a68925676669f111fd30f421fbe0.png)  
I would highly appreciate it if you could direct me to where this problem might arise from? Is there a specific file you believe I should inspect?  
I have also ensured that I have adequate permissions to the humann-3.5 folder (r/w/x).

Any insight you could provide will be much appreciated!

Thanks!