# Time of analysis questions **URL:** \ **Category:** HUMAnN\ **Created:** [October 8, 2021, 5:33pm UTC](https://forum.biobakery.org/t/time-of-analysis-questions/2676 "2021-10-08T17:33:59Z")\ **Posts on this page:** 3\ **Page:** 1
**Author:** ![Carla\_Uranga](https://yyz1.discourse-cdn.com/flex027/user_avatar/forum.biobakery.org/carla_uranga/32/273_2.png) [@Carla\_Uranga](https://forum.biobakery.org/u/Carla_Uranga)\ **Post date:** [October 8, 2021, 5:33pm UTC](https://forum.biobakery.org/t/time-of-analysis-questions/2676/1 "2021-10-08T17:33:59Z")
Hi, I am able to get humann3 up and running, however, our fastq files are quite large (10-12 GB each), and we have 24 samples. Would you know if this would take hours or days using 40 cores (threads)? Thank you! Best wishes, Carla Uranga ---
**Author:** ![franzosa](https://yyz1.discourse-cdn.com/flex027/user_avatar/forum.biobakery.org/franzosa/32/3511_2.png) [@franzosa](https://forum.biobakery.org/u/franzosa)\ **Post date:** [October 14, 2021, 8:12pm UTC](https://forum.biobakery.org/t/time-of-analysis-questions/2676/2 "2021-10-14T20:12:20Z")
That’s pretty similar to the size of the files we used in benchmarking HUMAnN 3 (30M 150 nt reads). Depending on the settings, those ran for 8-16 hours using 5 cores, almost all of which is spent during translated search. I have found that performance doesn’t necessarily scale linearly with the number of cores per sample, such that you’re probably better off running e.g. 8 samples in parallel with 5 cores each vs. 1 sample at a time with 40 cores. ---
**Author:** ![y3zhu](https://yyz1.discourse-cdn.com/flex027/user_avatar/forum.biobakery.org/y3zhu/32/1080_2.png) [@y3zhu](https://forum.biobakery.org/u/y3zhu)\ **Post date:** [December 11, 2021, 6:33pm UTC](https://forum.biobakery.org/t/time-of-analysis-questions/2676/3 "2021-12-11T18:33:09Z")
> [@franzosa](#): > > 8 samples in parallel with 5 cores each Hi, i’m having similar issues. Can I ask how exactly do you achieve this? Thank you