# Shortbred identify performance with CARD and Uniref90

**URL:** <https://forum.biobakery.org/t/shortbred-identify-performance-with-card-and-uniref90/797>\
**Category:** ShortBRED\
**Created:** [July 30, 2020, 10:00am UTC](https://forum.biobakery.org/t/shortbred-identify-performance-with-card-and-uniref90/797 "2020-07-30T10:00:14Z")\
**Posts on this page:** 1\
**Page:** 1

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**Author:** ![Erwan](https://avatars.discourse-cdn.com/v4/letter/e/b4bc9f/32.png) [@Erwan](https://forum.biobakery.org/u/Erwan)\
**Post date:** [July 30, 2020, 10:00am UTC](https://forum.biobakery.org/t/shortbred-identify-performance-with-card-and-uniref90/797/1 "2020-07-30T10:00:14Z")

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Hi,

I want to create a set of markers with shortbred-identify using the newly released [CARD database (May 2020 version)](https://card.mcmaster.ca/download) as my proteins of interest and Uniref90 as my reference proteins sequences.

It seems that the process is going to take a while as my first try wasn’t over after three weeks. So my questions are :

1. Do you already have those markers created and available (such as the [mid-2017 ones](https://huttenhower.sph.harvard.edu/shortbred)) ?
2. Do you have any rough idea about how long it can take ?
3. Is there any way to speed up the process ? What about splitting the CARD fasta file and running multiple processes on a computer grid (i.e. one process per split fasta) ?

Thanks,  
Erwan