# Relative abundance multiply total sequence counts equals read counts?

**URL:** <https://forum.biobakery.org/t/relative-abundance-multiply-total-sequence-counts-equals-read-counts/1234>\
**Category:** MetaPhlAn\
**Created:** [November 5, 2020, 2:46pm UTC](https://forum.biobakery.org/t/relative-abundance-multiply-total-sequence-counts-equals-read-counts/1234 "2020-11-05T14:46:01Z")\
**Posts on this page:** 3\
**Page:** 1

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**Author:** ![CK\_zhu](https://yyz1.discourse-cdn.com/flex027/user_avatar/forum.biobakery.org/ck_zhu/32/151_2.png) [@CK\_zhu](https://forum.biobakery.org/u/CK_zhu)\
**Post date:** [November 5, 2020, 2:46pm UTC](https://forum.biobakery.org/t/relative-abundance-multiply-total-sequence-counts-equals-read-counts/1234/1 "2020-11-05T14:46:01Z")

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I saw an article from “nature comunication” said that relative abundance multiplied by total sequencing counts equals raw counts ?

Is this right？

 ![image](https://canada1.discourse-cdn.com/flex027/uploads/biobakery/original/1X/5767aad087a3d34a420e95c42327abed6c59ef12.png)

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**Author:** ![fbeghini](https://yyz1.discourse-cdn.com/flex027/user_avatar/forum.biobakery.org/fbeghini/32/81_2.png) [@fbeghini](https://forum.biobakery.org/u/fbeghini)\
**Post date:** [November 20, 2020, 11:01am UTC](https://forum.biobakery.org/t/relative-abundance-multiply-total-sequence-counts-equals-read-counts/1234/2 "2020-11-20T11:01:23Z")

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Predicted read counts can be also retrieved by running the analysis with `-t rel_ab_w_read_stats`

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**Author:** ![wusan1234](https://avatars.discourse-cdn.com/v4/letter/w/85e7bf/32.png) [@wusan1234](https://forum.biobakery.org/u/wusan1234)\
**Post date:** [August 25, 2022, 2:04am UTC](https://forum.biobakery.org/t/relative-abundance-multiply-total-sequence-counts-equals-read-counts/1234/3 "2022-08-25T02:04:18Z")

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thx for @fbeghini help,  
I noticed that this paper then use the read counts for humann, I find my metaphlan & humann results were both abundance files. If I want the readcount humann results, should I use the readcounts metaphlan feed to humann or any other method can transfer humann abundance files to readcount

thx!