# Only one Class in Plot Lefse Results

**URL:** <https://forum.biobakery.org/t/only-one-class-in-plot-lefse-results/4131>\
**Category:** LEfSe\
**Created:** [September 28, 2022, 7:47am UTC](https://forum.biobakery.org/t/only-one-class-in-plot-lefse-results/4131 "2022-09-28T07:47:20Z")\
**Posts on this page:** 2\
**Page:** 1

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**Author:** ![Kiran](https://yyz1.discourse-cdn.com/flex027/user_avatar/forum.biobakery.org/kiran/32/1680_2.png) [@Kiran](https://forum.biobakery.org/u/Kiran)\
**Post date:** [September 28, 2022, 7:47am UTC](https://forum.biobakery.org/t/only-one-class-in-plot-lefse-results/4131/1 "2022-09-28T07:47:20Z")

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Hi all,  
I am having a relative abundance files with two classes, samples no subclasses. When i run Lefse in Galaxy Site, I am getting only the features from one class instead of two. I am attaching the input files and the plot results figure for you reference.

 ![Cladogram](https://canada1.discourse-cdn.com/flex027/uploads/biobakery/original/2X/f/f0a68b5c7f6d0e69964b6638c32d93e7c826f4e8.png)  
 ![Lefse](https://canada1.discourse-cdn.com/flex027/uploads/biobakery/original/2X/e/e93e23634e3839eb6ee79357a15b7966473cc26c.png)

[Lefse.tsv](https://forum.biobakery.org/uploads/short-url/hRqE5ICbpyMdSzMOl31OHPk38TI.tsv) (88.5 KB)

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**Author:** ![mishort](https://yyz1.discourse-cdn.com/flex027/user_avatar/forum.biobakery.org/mishort/32/556_2.png) [@mishort](https://forum.biobakery.org/u/mishort)\
**Post date:** [September 30, 2022, 2:49pm UTC](https://forum.biobakery.org/t/only-one-class-in-plot-lefse-results/4131/2 "2022-09-30T14:49:13Z")

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Hi Kiran,  
The interpretation there is that all of the differentially abundant features have higher abundance in the jss\_b1 group compared to the other group. If there was a feature that was significantly more abundant in the other group, it would show up as another color.  
I hope that helps!  
Best,  
Meg