# No full uniref90 db in humann4 **URL:** \ **Category:** HUMAnN\ **Created:** [February 28, 2026, 6:48am UTC](https://forum.biobakery.org/t/no-full-uniref90-db-in-humann4/8807 "2026-02-28T06:48:14Z")\ **Posts on this page:** 3\ **Page:** 1
**Author:** ![XiaoluLIU11](https://yyz1.discourse-cdn.com/flex027/user_avatar/forum.biobakery.org/xiaoluliu11/32/3586_2.png) [@XiaoluLIU11](https://forum.biobakery.org/u/XiaoluLIU11)\ **Post date:** [February 28, 2026, 6:48am UTC](https://forum.biobakery.org/t/no-full-uniref90-db-in-humann4/8807/1 "2026-02-28T06:48:14Z")
I submitted a question on the forum: Currently using humann version 4.0.0a1. While downloading the database, I noticed the available databases are as follows: HUMAnN Databases ( database : build = location ) chocophlan : full = [http://huttenhower.sph.harvard.edu/humann\_data/chocophlan/chocophlan.v4\_alpha.tar.gz](http://huttenhower.sph.harvard.edu/humann_data/chocophlan/chocophlan.v4_alpha.tar.gz) chocophlan : ec\_filtered = [http://huttenhower.sph.harvard.edu/humann\_data/chocophlan/chocophlan\_EC\_FILTERED.v4\_alpha.tar.gz](http://huttenhower.sph.harvard.edu/humann_data/chocophlan/chocophlan_EC_FILTERED.v4_alpha.tar.gz) uniref : uniref90\_ec\_filtered\_diamond = [http://huttenhower.sph.harvard.edu/humann\_data/uniprot/uniref\_ec\_filtered/uniref90\_annotated\_v4\_alpha\_ec\_filtered.tar.gz](http://huttenhower.sph.harvard.edu/humann_data/uniprot/uniref_ec_filtered/uniref90_annotated_v4_alpha_ec_filtered.tar.gz) utility\_mapping : full = [http://huttenhower.sph.harvard.edu/humann\_data/full\_mapping\_v4\_alpha.tar.gz](http://huttenhower.sph.harvard.edu/humann_data/full_mapping_v4_alpha.tar.gz) Among these, uniref only offers uniref90\_ec\_filtered\_diamond. I noticed the tutorial recommends downloading uniref90\_diamond, but it seems this is not valid for humann4. Since the 2019 version is too old, but the current uniref90 is also a filtered version, is there a suitable full version of uniref90 for humann4? ---
**Author:** ![XiaoluLIU11](https://yyz1.discourse-cdn.com/flex027/user_avatar/forum.biobakery.org/xiaoluliu11/32/3586_2.png) [@XiaoluLIU11](https://forum.biobakery.org/u/XiaoluLIU11)\ **Post date:** [February 28, 2026, 7:24am UTC](https://forum.biobakery.org/t/no-full-uniref90-db-in-humann4/8807/2 "2026-02-28T07:24:16Z")
**Update on this issue:** I later checked the HUMAnN 4.0.0.alpha.1 release notes and confirmed that there is indeed no full version of UniRef90 available yet, only the filtered version (uniref90\_ec\_filtered\_diamond). It seems we’ll need to wait for the official release to get the complete version. **New issue encountered:** When running humann, I’m getting the following error: CRITICAL ERROR: Can not call software version for metaphlan However, I’ve confirmed that metaphlan is working properly: (biobakery) $ metaphlan -v MetaPhlAn version 4.2.4 (21 Oct 2025) Installed databases: mpa\_vJan25\_CHOCOPhlAnSGB\_202503 My environment details: - Using a biobakery environment created with conda/mamba - MetaPhlAn database installed in the default mamba environment location - HUMAnN version: 4.0.0.alpha.1 - MetaPhlAn version: 4.2.4 Does anyone know what might be causing this “Can not call software version for metaphlan” error? Could this be a compatibility issue between HUMAnN 4.0.0.alpha.1 and the latest MetaPhlAn 4.2.4? Looking at the release notes, HUMAnN 4.0.0.alpha.1 requires MetaPhlAn 4.0 and a specific database version (mpa\_vOct22\_CHOCOPhlAnSGB\_202403), but I have newer versions installed. Could this be the problem? ---
**Author:** ![Longhao\_Jia](https://yyz1.discourse-cdn.com/flex027/user_avatar/forum.biobakery.org/longhao_jia/32/3610_2.png) [@Longhao\_Jia](https://forum.biobakery.org/u/Longhao_Jia)\ **Post date:** [March 30, 2026, 2:11am UTC](https://forum.biobakery.org/t/no-full-uniref90-db-in-humann4/8807/3 "2026-03-30T02:11:16Z")
I get the same error. No full uniref90 db in humann4. ```auto (HUMAnN4) [jialonghao@admin databases]$ humann_databases --download uniref uniref90_diamond HUMAnN4_database ERROR: Please select an available build. (HUMAnN4) [jialonghao@admin databases]$ humann_databases -help usage: humann_databases [-h] [--available] [--download ] [--update-config {yes,no}] [--database-location DATABASE_LOCATION] HUMAnN Databases options: -h, --help show this help message and exit --available print the available databases --download download the selected database to the install location --update-config {yes,no} update the config file to set the new database as the default [DEFAULT: yes] --database-location DATABASE_LOCATION location (local or remote) to pull the database (HUMAnN4) [jialonghao@admin databases]$ humann_databases --available HUMAnN Databases ( database : build = location ) chocophlan : full = http://huttenhower.sph.harvard.edu/humann_data/chocophlan/chocophlan.v4_alpha.tar.gz chocophlan : ec_filtered = http://huttenhower.sph.harvard.edu/humann_data/chocophlan/chocophlan_EC_FILTERED.v4_alpha.tar.gz uniref : uniref90_ec_filtered_diamond = http://huttenhower.sph.harvard.edu/humann_data/uniprot/uniref_ec_filtered/uniref90_annotated_v4_alpha_ec_filtered.tar.gz utility_mapping : full = http://huttenhower.sph.harvard.edu/humann_data/full_mapping_v4_alpha.tar.gz (HUMAnN4) [jialonghao@admin databases]$ humann_databases --download uniref uniref90_ec_filtered_diamond HUMAnN4_database Creating subdirectory to install database: /public/home/jialonghao/GMMdb/databases/HUMAnN4_database/uniref Download URL: http://huttenhower.sph.harvard.edu/humann_data/uniprot/uniref_ec_filtered/uniref90_annotated_v4_alpha_ec_filtered.tar.gz 0.87 GB Extracting: /public/home/jialonghao/GMMdb/databases/HUMAnN4_database/uniref90_annotated_v4_alpha_ec_filtered.tar.gz Database installed: /public/home/jialonghao/GMMdb/databases/HUMAnN4_database/uniref HUMAnN configuration file updated: database_folders : protein = /public/home/jialonghao/GMMdb/databases/HUMAnN4_database/uniref ```