# Multiple comparison correcting

**URL:** https://forum.biobakery.org/t/multiple-comparison-correcting/258
**Category:** LEfSe
**Created:** [March 6, 2020, 5:11pm UTC](https://forum.biobakery.org/t/multiple-comparison-correcting/258 "2020-03-06T17:11:41Z")
**Posts on this page:** 1
**Showing post:** 4

<div class="post-metadata">

### Author: ![darmecian](https://avatars.discourse-cdn.com/v4/letter/d/47e85d/32.png) [@darmecian](https://forum.biobakery.org/u/darmecian)
#### Post date: [April 22, 2020, 9:02am UTC](https://forum.biobakery.org/t/multiple-comparison-correcting/258/4 "2020-04-22T09:02:51Z")

</div>

Hello Siyuan,

Thank you very much for the help! I understand what you mean.  
One more question is that, if we use KW test, and also perform LDA (with some threshold), is it still needed to perform multiple comparison control?

As you stated in the other thread, If those features passed those two test, I think these are considered to be biological biomarkers even if not performed multiple comparison correction.

> [@Lefse without any subclass- still valid?](https://forum.biobakery.org/t/lefse-without-any-subclass-still-valid/233/5):
>
> Hi there,  
> I’d agree with darmecian here. For 1, LEfSe will still perform Wilcoxon unless otherwise specified. For 2, I’d say so. Even in the absence of subclass tests LEfSe still filters for features that a) pass the KW test and b) has strong LDA score support. The two together should provide enough evidence for biomarkers.  
> Thanks,  
> Siyuan

---

_[View the full topic](https://forum.biobakery.org/t/multiple-comparison-correcting/258)._