# MinPath12hmp.py generates an error and Pathways are not created

**URL:** <https://forum.biobakery.org/t/minpath12hmp-py-generates-an-error-and-pathways-are-not-created/7101>\
**Category:** HUMAnN\
**Created:** [May 29, 2024, 9:46am UTC](https://forum.biobakery.org/t/minpath12hmp-py-generates-an-error-and-pathways-are-not-created/7101 "2024-05-29T09:46:13Z")\
**Posts on this page:** 1\
**Page:** 1

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**Author:** ![Vadim\_Dubinsky](https://yyz1.discourse-cdn.com/flex027/user_avatar/forum.biobakery.org/vadim_dubinsky/32/356_2.png) [@Vadim\_Dubinsky](https://forum.biobakery.org/u/Vadim_Dubinsky)\
**Post date:** [May 29, 2024, 9:46am UTC](https://forum.biobakery.org/t/minpath12hmp-py-generates-an-error-and-pathways-are-not-created/7101/1 "2024-05-29T09:46:13Z")

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Dear developers and users,

I am using the new ver of HUMAnN3 (v3.9) coupled with MetaPhlan4 (v4.1.1).  
For humann I am using chocophlan.v201901\_v31 and uniref90\_201901b dbs, and for metaphlan the latest vJun23 db.

HUMAnN3 generates an error on every run, only the genefamilies output is created.  
The error is in MinPath script (in short):

```auto
humann.utilities - CRITICAL: Error executing: /home/ubuntu/mambaforge/bin/python3.10 /home/ubuntu/mambaforge/lib/python3.10/site-packages/humann/quantify/MinPath12hmp.py ...

```

```auto
humann.utilities - CRITICAL: TRACEBACK:
Traceback (most recent call last):
  File "/home/ubuntu/mambaforge/lib/python3.10/site-packages/humann/utilities.py", line 759, in execute_command
    p = subprocess.check_call(cmd, stdin=stdin, stdout=stdout, stderr=stderr)
  File "/home/ubuntu/mambaforge/lib/python3.10/subprocess.py", line 369, in check_call
    raise CalledProcessError(retcode, cmd)
subprocess.CalledProcessError: ... returned non-zero exit status 1.

```

Is there some problem with compatibility between some of the dbs I am using? I am feeding HUMAnN3 the species profile obtained from MetaPhlan4.  
As far as I understand the problem is in metacyc pathways creation, and those dbs are not directly related to it.

Any help will be appreciated!  
VadimD