# Metaphlan4 no eukaryotes

**URL:** <https://forum.biobakery.org/t/metaphlan4-no-eukaryotes/4920>\
**Category:** MetaPhlAn\
**Created:** [March 5, 2023, 10:38pm UTC](https://forum.biobakery.org/t/metaphlan4-no-eukaryotes/4920 "2023-03-05T22:38:16Z")\
**Posts on this page:** 3\
**Page:** 1

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**Author:** ![Theo\_Allnutt](https://yyz1.discourse-cdn.com/flex027/user_avatar/forum.biobakery.org/theo_allnutt/32/2011_2.png) [@Theo\_Allnutt](https://forum.biobakery.org/u/Theo_Allnutt)\
**Post date:** [March 5, 2023, 10:38pm UTC](https://forum.biobakery.org/t/metaphlan4-no-eukaryotes/4920/1 "2023-03-05T22:38:16Z")

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Hi,

is there any reason why Metaphlan4 is not profiling eukaryotes? I have samples that showed eukaryotes in Metaphlan2 but now they are absent.

Thanks,

Theo

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<div class="post-metadata">

**Author:** ![Theo\_Allnutt](https://yyz1.discourse-cdn.com/flex027/user_avatar/forum.biobakery.org/theo_allnutt/32/2011_2.png) [@Theo\_Allnutt](https://forum.biobakery.org/u/Theo_Allnutt)\
**Post date:** [March 5, 2023, 11:45pm UTC](https://forum.biobakery.org/t/metaphlan4-no-eukaryotes/4920/2 "2023-03-05T23:45:08Z")

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I also note there are no viruses.

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**Author:** ![jolespin](https://yyz1.discourse-cdn.com/flex027/user_avatar/forum.biobakery.org/jolespin/32/1838_2.png) [@jolespin](https://forum.biobakery.org/u/jolespin)\
**Post date:** [March 7, 2023, 8:25pm UTC](https://forum.biobakery.org/t/metaphlan4-no-eukaryotes/4920/3 "2023-03-07T20:25:30Z")

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I haven’t tested yet but I second this as a useful inclusion. Especially for usage in marine systems. Would aligning to the BUSCO eukaryota\_odb10 be a good usage for markers to use? Obviously the ids would have to be worked backwards to get the CDS.