# Metaphlan profile not showing any relative abundance

**URL:** <https://forum.biobakery.org/t/metaphlan-profile-not-showing-any-relative-abundance/212>\
**Category:** MetaPhlAn\
**Created:** [February 15, 2020, 9:54pm UTC](https://forum.biobakery.org/t/metaphlan-profile-not-showing-any-relative-abundance/212 "2020-02-15T21:54:56Z")\
**Posts on this page:** 3\
**Page:** 1

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**Author:** ![DEEPCHANDA7](https://avatars.discourse-cdn.com/v4/letter/d/eb9ed0/32.png) [@DEEPCHANDA7](https://forum.biobakery.org/u/DEEPCHANDA7)\
**Post date:** [February 15, 2020, 9:54pm UTC](https://forum.biobakery.org/t/metaphlan-profile-not-showing-any-relative-abundance/212/1 "2020-02-15T21:54:56Z")

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I used prefetch tool of “SRA-TOOLKIT-2.9.6” was used to download SRR11048974 (.sra file). It was converted to .fastq file by fastq-dump of SRA-TOOLKIT. this .fastq file was analysed by “MetaPhlAn version 2.96.1 (02 Feb 2020)”. But both the “SRR11048974.fastq.bowtie2out.txt” and profile “SRR11048974.txt” files are not giving expected result. It shows:  
“#mpa\_v296\_CHOCOPhlAn\_201901  
#/home/deep/miniconda3/bin/metaphlan2.py SRR11048974.fastq --input\_type fastq --nproc 4  
#SampleID Metaphlan2\_Analysis  
#clade\_name NCBI\_tax\_id relative\_abundance  
UNKNOWN -1 100.0”

I am facing same output for many other files also.  
But i found good results when run the example samples given in tutorial link ([https://bitbucket.org/biobakery/biobakery/wiki/metaphlan2#rst-header-run-a-single-sample](https://bitbucket.org/biobakery/biobakery/wiki/metaphlan2#rst-header-run-a-single-sample)).  
I’m a newbie in this field. please help me in simple understandable language.

Thanks in advance.

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**Author:** ![franzosa](https://yyz1.discourse-cdn.com/flex027/user_avatar/forum.biobakery.org/franzosa/32/3511_2.png) [@franzosa](https://forum.biobakery.org/u/franzosa)\
**Post date:** [April 23, 2020, 5:53pm UTC](https://forum.biobakery.org/t/metaphlan-profile-not-showing-any-relative-abundance/212/2 "2020-04-23T17:53:06Z")

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Sorry we missed this thread earlier. SRR11048974 appears to be 16S rRNA amplicon sequencing data, whereas MetaPhlAn is designed for use with shotgun sequencing data.

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**Author:** ![DEEPCHANDA7](https://avatars.discourse-cdn.com/v4/letter/d/eb9ed0/32.png) [@DEEPCHANDA7](https://forum.biobakery.org/u/DEEPCHANDA7)\
**Post date:** [April 26, 2020, 12:49pm UTC](https://forum.biobakery.org/t/metaphlan-profile-not-showing-any-relative-abundance/212/3 "2020-04-26T12:49:54Z")

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Okay Franzosa, now I understand. Thanks a lot.