# Metaphlan\_16s\_Shotgun

**URL:** https://forum.biobakery.org/t/metaphlan-16s-shotgun/4997
**Category:** MetaPhlAn
**Created:** [March 21, 2023, 4:23am UTC](https://forum.biobakery.org/t/metaphlan-16s-shotgun/4997 "2023-03-21T04:23:29Z")
**Posts on this page:** 2
**Page:** 1

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### Author: ![Santhiya](https://avatars.discourse-cdn.com/v4/letter/s/ee7513/32.png) [@Santhiya](https://forum.biobakery.org/u/Santhiya)
#### Post date: [March 21, 2023, 4:23am UTC](https://forum.biobakery.org/t/metaphlan-16s-shotgun/4997/1 "2023-03-21T04:23:30Z")

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Is that possible to map 16s reads from Shotgun data using Metaphlan?

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### Author: ![aitor.blancomiguez](https://yyz1.discourse-cdn.com/flex027/user_avatar/forum.biobakery.org/aitor.blancomiguez/32/86_2.png) [@aitor.blancomiguez](https://forum.biobakery.org/u/aitor.blancomiguez)
#### Post date: [March 23, 2023, 9:07am UTC](https://forum.biobakery.org/t/metaphlan-16s-shotgun/4997/2 "2023-03-23T09:07:59Z")

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Hi @Santhiya  
Unfortunatelly not, Metaphlan 4 only maps reads from the species-specific (genes not present in any other species) marker genes of the species in the database