# Maaslin3 summary plot

**URL:** <https://forum.biobakery.org/t/maaslin3-summary-plot/8115>\
**Category:** MaAsLin\
**Created:** [June 7, 2025, 6:55am UTC](https://forum.biobakery.org/t/maaslin3-summary-plot/8115 "2025-06-07T06:55:38Z")\
**Posts on this page:** 2\
**Page:** 1

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**Author:** ![Chun\_Wie\_Chong](https://yyz1.discourse-cdn.com/flex027/user_avatar/forum.biobakery.org/chun_wie_chong/32/3281_2.png) [@Chun\_Wie\_Chong](https://forum.biobakery.org/u/Chun_Wie_Chong)\
**Post date:** [June 7, 2025, 6:55am UTC](https://forum.biobakery.org/t/maaslin3-summary-plot/8115/1 "2025-06-07T06:55:38Z")

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I have conducted subset analysis for 2 groups, Group A and Group B. For each group, there are 3 timepoints, W1, W4 and W8.

The analysis ran well, but I have issue with the summary plots.

For Group A, the order of the summary plot is W8 followed by W4. However, for Group B, the order is W4 followed by W8.

I have tried the following:

1. re-ran the analysis by setting “factor” for Week category
2. changed the full\_metadata\_name from the data extracted from the RDS object
3. set the factor for the all\_results.tsv and rerun using maaslin\_plot\_results\_from\_output

However, all failed. Any advice is appreciated? Thank you.

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**Author:** ![WillNickols](https://yyz1.discourse-cdn.com/flex027/user_avatar/forum.biobakery.org/willnickols/32/3223_2.png) [@WillNickols](https://forum.biobakery.org/u/WillNickols)\
**Post date:** [June 7, 2025, 12:23pm UTC](https://forum.biobakery.org/t/maaslin3-summary-plot/8115/2 "2025-06-07T12:23:00Z")

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Hi

If you look at `coef_plot_vars` and `heatmap_vars` in `?maaslin3::maaslin3`, do those do what you want?

Will
