# LEfSe"\*\* tool (Galaxy Version 1.0)

**URL:** <https://forum.biobakery.org/t/lefse-tool-galaxy-version-1-0/7852>\
**Category:** Data resource\
**Created:** [February 22, 2025, 12:23pm UTC](https://forum.biobakery.org/t/lefse-tool-galaxy-version-1-0/7852 "2025-02-22T12:23:40Z")\
**Posts on this page:** 2\
**Page:** 1

<div class="post-metadata">

**Author:** ![Khalid\_Ibrahim](https://yyz1.discourse-cdn.com/flex027/user_avatar/forum.biobakery.org/khalid_ibrahim/32/2437_2.png) [@Khalid\_Ibrahim](https://forum.biobakery.org/u/Khalid_Ibrahim)\
**Post date:** [February 22, 2025, 12:23pm UTC](https://forum.biobakery.org/t/lefse-tool-galaxy-version-1-0/7852/1 "2025-02-22T12:23:40Z")

</div>

I am attempting to use the **“Format Data for LEfSe”** tool (Galaxy Version 1.0) for my analysis. However, I am facing issues getting it to work properly. I even tried running the tool with the example dataset provided, such as `hmp_aerobiosis_small` , but it still doesn’t function as expected. Could you please provide guidance on how to resolve this issue?  
thank you in advance

Regrads

---

<div class="post-metadata">

**Author:** ![nataliauni](https://avatars.discourse-cdn.com/v4/letter/n/f1d935/32.png) [@nataliauni](https://forum.biobakery.org/u/nataliauni)\
**Post date:** [March 13, 2025, 7:47pm UTC](https://forum.biobakery.org/t/lefse-tool-galaxy-version-1-0/7852/2 "2025-03-13T19:47:00Z")

</div>

I’m also having the same issues, including formatting the example dataset. I would like to add that I was able to format the input of my data using command line but I couldn’t run LDA so that’s why I wanted to use galaxy but I wasn’t even able to perform step **A) Format Data for LEfSe** in galaxy
