# Kneaddata memory error

**URL:** <https://forum.biobakery.org/t/kneaddata-memory-error/1252>\
**Category:** KneadData\
**Created:** [November 10, 2020, 8:33pm UTC](https://forum.biobakery.org/t/kneaddata-memory-error/1252 "2020-11-10T20:33:12Z")\
**Posts on this page:** 1\
**Page:** 1

<div class="post-metadata">

**Author:** ![Robin\_Mjelle](https://yyz1.discourse-cdn.com/flex027/user_avatar/forum.biobakery.org/robin_mjelle/32/258_2.png) [@Robin\_Mjelle](https://forum.biobakery.org/u/Robin_Mjelle)\
**Post date:** [November 10, 2020, 8:33pm UTC](https://forum.biobakery.org/t/kneaddata-memory-error/1252/1 "2020-11-10T20:33:12Z")

</div>

I am getting the following error when running Kneaddata:

kneaddata -t 20 --input **$FILE**.1.fastq --input **$FILE**.2.fastq --reference-db ~/projects/Colorectal/Metagenomics --trimmomatic /mnt/scratch/home/local/robinm/miniconda2/share/trimmomatic --max-memory 200 --output **$FILE**

Traceback (most recent call last):  
File “/home/local/robinm/miniconda2/bin/kneaddata”, line 10, in   
sys.exit(main())  
File “/home/local/robinm/miniconda2/lib/python2.7/site-packages/kneaddata/knead\_data.py”, line 427, in main  
args.input=utilities.check\_and\_reorder\_reads(args.input, args.output\_dir, temp\_output\_files)  
File “/home/local/robinm/miniconda2/lib/python2.7/site-packages/kneaddata/utilities.py”, line 58, in check\_and\_reorder\_reads  
for lines, pair\_id in zip(read\_file\_n\_lines(input\_files[1],4), ids):  
MemoryError