# Issue while calculating LDA effect size

**URL:** <https://forum.biobakery.org/t/issue-while-calculating-lda-effect-size/2216>\
**Category:** LEfSe\
**Created:** [June 8, 2021, 5:51am UTC](https://forum.biobakery.org/t/issue-while-calculating-lda-effect-size/2216 "2021-06-08T05:51:02Z")\
**Posts on this page:** 2\
**Page:** 1

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**Author:** ![Ankush1](https://avatars.discourse-cdn.com/v4/letter/a/e47774/32.png) [@Ankush1](https://forum.biobakery.org/u/Ankush1)\
**Post date:** [June 8, 2021, 5:51am UTC](https://forum.biobakery.org/t/issue-while-calculating-lda-effect-size/2216/1 "2021-06-08T05:51:02Z")

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Hi all,

I am new to using LEfSe.

I was following the tutorials and old forums to use LEfSE and to resolve the errors.

Though, I have got stuck at the second step and getting the following output while calculating LDA Effect size:  
Number of significantly discriminative features: 0 ( 0 ) before internal wilcoxon  
No features with significant differences between the two classes  
Number of discriminative features with abs LDA score \> 2.0 : 0

I looked into previous forums where similar issuse was discussed but couldn’t help myself with this.

Attached is the data file I am working with.  
[feature-table.biom.txt](https://forum.biobakery.org/uploads/short-url/6myruG3OltxquTDmPeTymOKgUya.txt) (16.3 KB)

Could anyone please help me in getting through this step? Your help will be greatly appreciated. Thanks

Kind Regards  
Ankush

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**Author:** ![mishort](https://yyz1.discourse-cdn.com/flex027/user_avatar/forum.biobakery.org/mishort/32/556_2.png) [@mishort](https://forum.biobakery.org/u/mishort)\
**Post date:** [June 10, 2021, 2:26pm UTC](https://forum.biobakery.org/t/issue-while-calculating-lda-effect-size/2216/2 "2021-06-10T14:26:48Z")

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Hi Ankush,  
It simply means that after analyzing the input features, none of them were significantly different across levels of your group variable (in other words, no significant hits).  
Best,  
Meg