# Interpreting Output - one hit set for 2 proteins

**URL:** <https://forum.biobakery.org/t/interpreting-output-one-hit-set-for-2-proteins/3550>\
**Category:** ShortBRED\
**Created:** [May 6, 2022, 8:31pm UTC](https://forum.biobakery.org/t/interpreting-output-one-hit-set-for-2-proteins/3550 "2022-05-06T20:31:46Z")\
**Posts on this page:** 1\
**Page:** 1

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**Author:** ![bmtalbot](https://yyz1.discourse-cdn.com/flex027/user_avatar/forum.biobakery.org/bmtalbot/32/582_2.png) [@bmtalbot](https://forum.biobakery.org/u/bmtalbot)\
**Post date:** [May 6, 2022, 8:31pm UTC](https://forum.biobakery.org/t/interpreting-output-one-hit-set-for-2-proteins/3550/1 "2022-05-06T20:31:46Z")

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Hi there! I recently ran an analysis using AMR markers from CARD. In my final output table, I noticed that a single like had two predicted proteins:

gb YP\_039996\_1 **ARO\_3003285** Staphylococcus\_gb YP\_039996\_1 **ARO\_3003287** Staphylococcus

They code for different subunits of the same protein (rpoB if you’re curious!), and suggest two different mutations for resistance to different antibiotics. How should I interpret this outcome, if there is only one set of hits for both predictions? Is this considered that it was an either/or for the kmer group? Or is the group getting assigned as an aggregate? Is something else going on?

Thanks for your help!  
Brooke