# Internet connection? **URL:** \ **Category:** MetaPhlAn\ **Created:** [March 11, 2020, 5:19pm UTC](https://forum.biobakery.org/t/internet-connection/269 "2020-03-11T17:19:48Z")\ **Posts on this page:** 8\ **Page:** 1
**Author:** ![idapantoja](https://yyz1.discourse-cdn.com/flex027/user_avatar/forum.biobakery.org/idapantoja/32/144_2.png) [@idapantoja](https://forum.biobakery.org/u/idapantoja)\ **Post date:** [March 11, 2020, 5:19pm UTC](https://forum.biobakery.org/t/internet-connection/269/1 "2020-03-11T17:19:48Z")
Hi all! I have basic question about the use of Metaphlan2 in a standalone computer with no connection to internet. The software is installed in a Mac computer but can it worked without internet? Or internet connection is required to run all the analysis? I would appreciate the feedback on this. Thank you! idapantoja ---
**Author:** ![fbeghini](https://yyz1.discourse-cdn.com/flex027/user_avatar/forum.biobakery.org/fbeghini/32/81_2.png) [@fbeghini](https://forum.biobakery.org/u/fbeghini)\ **Post date:** [March 12, 2020, 5:07pm UTC](https://forum.biobakery.org/t/internet-connection/269/2 "2020-03-12T17:07:49Z")
Hi, the internet connection is required only for downloading the database. The analysis can be done offline. ---
**Author:** ![idapantoja](https://yyz1.discourse-cdn.com/flex027/user_avatar/forum.biobakery.org/idapantoja/32/144_2.png) [@idapantoja](https://forum.biobakery.org/u/idapantoja)\ **Post date:** [March 12, 2020, 5:25pm UTC](https://forum.biobakery.org/t/internet-connection/269/3 "2020-03-12T17:25:34Z")
Hi, Perfect! Thanks so much for the answer! ---
**Author:** ![DEEPCHANDA7](https://avatars.discourse-cdn.com/v4/letter/d/eb9ed0/32.png) [@DEEPCHANDA7](https://forum.biobakery.org/u/DEEPCHANDA7)\ **Post date:** [March 25, 2020, 8:13pm UTC](https://forum.biobakery.org/t/internet-connection/269/4 "2020-03-25T20:13:01Z")
Hi Fransesco, I was trying to download clade marker database locally by the command: `$ metaphlan2.py --install` My network download speed is not less than at least 1.5MBps. But when I download this particular database, it becomes super slow (around 20kbps). Is it normal or I’m having some issues? ---
**Author:** ![idapantoja](https://yyz1.discourse-cdn.com/flex027/user_avatar/forum.biobakery.org/idapantoja/32/144_2.png) [@idapantoja](https://forum.biobakery.org/u/idapantoja)\ **Post date:** [July 16, 2020, 8:09pm UTC](https://forum.biobakery.org/t/internet-connection/269/5 "2020-07-16T20:09:23Z")
Hi! Hope you are doing well! I am retaking this topic again. I was not able to work on this due to the pandemic. So my issue is the following. I was trying the command: metaphlan2.py Natick-WGS-240\_R1.fastq --input\_type fasta \> Natick\_WGS\_240 (that is the name of the sample I am using as practice) and I was getting this message: metaphla2.py: command not found I started looking on internet and I found that you could add python at the beginning like this: python metaphlan2.py Natick-WGS-240\_R1.fastq --input\_type fasta \> Natick\_WGS\_240 I got this after running that option: Warning! Biom python library not detected! Exporting to biom format will not work ! Downloading MetaPhlan2 database Please note due to the size this might take a few minutes Downloading [https://bitbucket.org/biobakery/metaphlan2/downloads/mpa\_v20\_m200.tar](https://bitbucket.org/biobakery/metaphlan2/downloads/mpa_v20_m200.tar) Warning: Unable to download [https://bitbucket.org/biobakery/metaphlan2/downloads/mpa\_v20\_m200.tar](https://bitbucket.org/biobakery/metaphlan2/downloads/mpa_v20_m200.tar) Downloading [https://bitbucket.org/biobakery/metaphlan2/downloads/mpa\_v20\_m200.md5](https://bitbucket.org/biobakery/metaphlan2/downloads/mpa_v20_m200.md5) Warning: Unable to download Downloading [https://bitbucket.org/biobakery/metaphlan2/downloads/mpa\_v20\_m200.md5](https://bitbucket.org/biobakery/metaphlan2/downloads/mpa_v20_m200.md5) File “/Users/ida.pantojafeliciano/Downloads/metaphlan2/metaphalan\_databases/mpa\_v20\_m200.md5” not found! File “/Users/ida.pantojafeliciano/Downloads/metaphlan2/metaphalan\_databases/mpa\_v20\_m200.tar” not found! MD5 checksums not found, something went wrong! I got a folder called “metaphlan\_databases” but is empty. So this is the step that requires internet? I checked and I can’t find mpa\_v20\_m200.tar and mpa\_v20\_m200.md5 in any place in the computer. Its a good idea to use “python” at the beginning of the command? Thank you! Ida ---
**Author:** ![fbeghini](https://yyz1.discourse-cdn.com/flex027/user_avatar/forum.biobakery.org/fbeghini/32/81_2.png) [@fbeghini](https://forum.biobakery.org/u/fbeghini)\ **Post date:** [July 20, 2020, 8:38am UTC](https://forum.biobakery.org/t/internet-connection/269/6 "2020-07-20T08:38:41Z")
Yes, in order to install the database, an internet connection is required. But first, you should update your MetaPhlAn2 installation, if you have conda installed, you can run `conda install metaphlan2=2.7.8` since the local copy you have is an old build still pointing to the old repo. Then, you should run `metaphlan2.py --install` for downloading and builiding the database. ---
**Author:** ![idapantoja](https://yyz1.discourse-cdn.com/flex027/user_avatar/forum.biobakery.org/idapantoja/32/144_2.png) [@idapantoja](https://forum.biobakery.org/u/idapantoja)\ **Post date:** [July 20, 2020, 6:48pm UTC](https://forum.biobakery.org/t/internet-connection/269/7 "2020-07-20T18:48:32Z")
Hi @fbeghini, Thanks so much for your answer. I am guessing that for the conda install command I need the internet connection too, right? Best regards! @idapantoja ---
**Author:** ![fbeghini](https://yyz1.discourse-cdn.com/flex027/user_avatar/forum.biobakery.org/fbeghini/32/81_2.png) [@fbeghini](https://forum.biobakery.org/u/fbeghini)\ **Post date:** [July 22, 2020, 7:32am UTC](https://forum.biobakery.org/t/internet-connection/269/8 "2020-07-22T07:32:12Z")
Yes, unfortunately, yes. To avoid using conda, you can clone the repository from GitHub and manually download the database from [here](https://drive.google.com/drive/folders/1_HaY16mT7mZ_Z8JtesH8zCfG9ikWcLXG?usp=sharing)