# Including/Excluding ;\_\_

**URL:** <https://forum.biobakery.org/t/including-excluding/6339>\
**Category:** LEfSe\
**Created:** [December 18, 2023, 10:10pm UTC](https://forum.biobakery.org/t/including-excluding/6339 "2023-12-18T22:10:30Z")\
**Posts on this page:** 1\
**Page:** 1

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**Author:** ![capsola](https://yyz1.discourse-cdn.com/flex027/user_avatar/forum.biobakery.org/capsola/32/2200_2.png) [@capsola](https://forum.biobakery.org/u/capsola)\
**Post date:** [December 18, 2023, 10:10pm UTC](https://forum.biobakery.org/t/including-excluding/6339/1 "2023-12-18T22:10:30Z")

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Hi! Just a curiosity question I could not seem to find on the forum. One of my coworkers has found that when modifying the L7 relative abundance file for lefse, including or excluding the end ;\_\_ on the ASV nanes yields different results (significant with, not significant without). I assume that excluding the ;\_\_ on our ASVs is important, but I’m unsure the specific reason why.

Thank you!