# I am not getting viruses abundance in MetaPhlAn4.1 using mpa\_vJun23 database

**URL:** <https://forum.biobakery.org/t/i-am-not-getting-viruses-abundance-in-metaphlan4-1-using-mpa-vjun23-database/6749>\
**Category:** MetaPhlAn\
**Created:** [March 1, 2024, 11:23pm UTC](https://forum.biobakery.org/t/i-am-not-getting-viruses-abundance-in-metaphlan4-1-using-mpa-vjun23-database/6749 "2024-03-01T23:23:56Z")\
**Posts on this page:** 2\
**Page:** 1

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**Author:** ![rohitshukla](https://avatars.discourse-cdn.com/v4/letter/r/898d66/32.png) [@rohitshukla](https://forum.biobakery.org/u/rohitshukla)\
**Post date:** [March 1, 2024, 11:23pm UTC](https://forum.biobakery.org/t/i-am-not-getting-viruses-abundance-in-metaphlan4-1-using-mpa-vjun23-database/6749/1 "2024-03-01T23:23:56Z")

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Hey,  
Thanks for releasing the MetaPhlAn 4.1. It will be a great improvement. However when I am trying to find the virus from this version, so i am not able to find it. However, with Metaphlan 3.0, I can find 5% of viruses in my sample.

I downloaded the database mpa\_vJun23 using the Metaplan download option. Then I am trying to find the viruses through these commands.

metaphlan Gut\_MG0182\_S52\_L001\_R1\_001.fastq.gz --input\_type fastq -o profiled\_metagenome\_3.txt --nproc 12 --bowtie2db /mnt/d/metaphlan\_4.1\_db/ --add\_viruses --mpa3 --profile\_vsc --ignore\_bacteria --ignore\_archaea

metaphlan Gut\_MG0182\_S52\_L001\_R1\_001.fastq.gz --input\_type fastq -o profiled\_metagenome\_3.txt --nproc 12 --bowtie2db /mnt/d/metaphlan\_db --add\_viruses --mpa3 --profile\_vsc --ignore\_bacteria --ignore\_archaea

metaphlan Gut\_MG0182\_S52\_L001\_R1\_001.fastq.gz --input\_type fastq -o profiled\_metagenome.txt --profile\_vsc --nproc 12 --bowtie2db /mnt/d/metaphlan\_4.1\_db/

I can successfully get the Archea and bacteria but cannot get the virus in the same fastq file where I am getting 5% viral reads from Metaphlan3.

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**Author:** ![aitor.blancomiguez](https://yyz1.discourse-cdn.com/flex027/user_avatar/forum.biobakery.org/aitor.blancomiguez/32/86_2.png) [@aitor.blancomiguez](https://forum.biobakery.org/u/aitor.blancomiguez)\
**Post date:** [March 5, 2024, 1:08pm UTC](https://forum.biobakery.org/t/i-am-not-getting-viruses-abundance-in-metaphlan4-1-using-mpa-vjun23-database/6749/2 "2024-03-05T13:08:04Z")

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Hi @rohitshukla  
In version 4.1, the viral profiling is reported in an additional individual file, please, have a look at the tutorial here: [MetaPhlAn 4.1 · biobakery/biobakery Wiki · GitHub](https://github.com/biobakery/biobakery/wiki/MetaPhlAn-4.1#3-metaphlan-viral-module-tutorial)