# HUMAnN3 diamond uniref problem

**URL:** <https://forum.biobakery.org/t/humann3-diamond-uniref-problem/618>\
**Category:** HUMAnN\
**Created:** [June 20, 2020, 10:33pm UTC](https://forum.biobakery.org/t/humann3-diamond-uniref-problem/618 "2020-06-20T22:33:04Z")\
**Posts on this page:** 2\
**Page:** 1

<div class="post-metadata">

**Author:** ![nankaiwolf](https://avatars.discourse-cdn.com/v4/letter/n/5f8ce5/32.png) [@nankaiwolf](https://forum.biobakery.org/u/nankaiwolf)\
**Post date:** [June 20, 2020, 10:33pm UTC](https://forum.biobakery.org/t/humann3-diamond-uniref-problem/618/1 "2020-06-20T22:33:04Z")

</div>

In HUMAnN3 I use diamond 0.9.24.125 to align uniref databases: uniref50\_annotated\_v201901, uniref50\_ec\_filtered\_v201901, uniref90\_annotated\_v201901. However, uniref90\_ec\_filtered\_v201901 does not work out. It is said, Database was built with a different version of Diamond and is incompatible.  
So which version of diamond do I choose to use?

---

<div class="post-metadata">

**Author:** ![franzosa](https://yyz1.discourse-cdn.com/flex027/user_avatar/forum.biobakery.org/franzosa/32/3511_2.png) [@franzosa](https://forum.biobakery.org/u/franzosa)\
**Post date:** [June 24, 2020, 3:09pm UTC](https://forum.biobakery.org/t/humann3-diamond-uniref-problem/618/2 "2020-06-24T15:09:37Z")

</div>

Hmm, that’s an error that you get if you use a DIAMOND 0.8 database with DIAMOND 0.9 (or vice versa). The v201901 databases should all be built for DIAMOND 0.9 only. Are you sure that the diamond you are using for alignment in 0.9? Can you do a `diamond --version` check?