# Humann2 input file

**URL:** <https://forum.biobakery.org/t/humann2-input-file/516>\
**Category:** HUMAnN\
**Created:** [May 26, 2020, 10:58am UTC](https://forum.biobakery.org/t/humann2-input-file/516 "2020-05-26T10:58:36Z")\
**Posts on this page:** 2\
**Page:** 1

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**Author:** ![DEEPCHANDA7](https://avatars.discourse-cdn.com/v4/letter/d/eb9ed0/32.png) [@DEEPCHANDA7](https://forum.biobakery.org/u/DEEPCHANDA7)\
**Post date:** [May 26, 2020, 10:58am UTC](https://forum.biobakery.org/t/humann2-input-file/516/1 "2020-05-26T10:58:36Z")

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Hi friends!!! (@fbeghini@franzosa @f.asnicar )  
I have installed MetaPhlAn2 (version 2.96.1) from anaconda. I haven’t installed HUMAnN2 yet. I have already profiled some of my WGS fastq files and got outputs files.  
Can I install Humann2 separately and use these output files there? If yes. How?  
Or, I must install Humann2 separately with integrated Metaphlan2 and again start with the WGS fastq files?

Thanks & Regards,  
DC7

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**Author:** ![franzosa](https://yyz1.discourse-cdn.com/flex027/user_avatar/forum.biobakery.org/franzosa/32/3511_2.png) [@franzosa](https://forum.biobakery.org/u/franzosa)\
**Post date:** [May 29, 2020, 6:43pm UTC](https://forum.biobakery.org/t/humann2-input-file/516/2 "2020-05-29T18:43:56Z")

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It is better to use an earlier MetaPhlAn with HUMAnN 2.0 _or_ use the 3.0 versions of both tools, as described here:

[https://forum.biobakery.org/t/announcing-humann-3-0-alpha/518/2](https://forum.biobakery.org/t/announcing-humann-3-0-alpha/518/2)

If you already have a taxonomic profile from MetaPhlAn you can pass it to HUMAnN via the `--tax-profile` flag (i.e. you don’t need to rerun MetaPhlAn as the normal first step of HUMAnN).