# HuManN v4 output are different

**URL:** <https://forum.biobakery.org/t/humann-v4-output-are-different/7218>\
**Category:** HUMAnN\
**Created:** [July 3, 2024, 7:12am UTC](https://forum.biobakery.org/t/humann-v4-output-are-different/7218 "2024-07-03T07:12:47Z")\
**Posts on this page:** 6\
**Page:** 1

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**Author:** ![Michela\_Francesconi](https://yyz1.discourse-cdn.com/flex027/user_avatar/forum.biobakery.org/michela_francesconi/32/2874_2.png) [@Michela\_Francesconi](https://forum.biobakery.org/u/Michela_Francesconi)\
**Post date:** [July 3, 2024, 7:12am UTC](https://forum.biobakery.org/t/humann-v4-output-are-different/7218/1 "2024-07-03T07:12:47Z")

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Hello to everyone!

I downloaded HuManN from the GitHub web site ([GitHub - biobakery/humann: HUMAnN is the next generation of HUMAnN 1.0 (HMP Unified Metabolic Analysis Network).](https://github.com/biobakery/humann)) so I should have the version number 3 but when I check (humann --version) I have the v4.0. Do you know why?

When I run my data the output are a bit different, I do not have the pathway coverage file but a reactions file.

Also when I look at my gene family file, I just have I species and a t\_ that I do not understand what is, where I can find the family and the gender?

Thank you!

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**Author:** ![Xin\_Li](https://yyz1.discourse-cdn.com/flex027/user_avatar/forum.biobakery.org/xin_li/32/2822_2.png) [@Xin\_Li](https://forum.biobakery.org/u/Xin_Li)\
**Post date:** [July 6, 2024, 2:37am UTC](https://forum.biobakery.org/t/humann-v4-output-are-different/7218/2 "2024-07-06T02:37:30Z")

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I had the same issue before. I think you should install through conda instead.

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**Author:** ![franzosa](https://yyz1.discourse-cdn.com/flex027/user_avatar/forum.biobakery.org/franzosa/32/3511_2.png) [@franzosa](https://forum.biobakery.org/u/franzosa)\
**Post date:** [July 16, 2024, 7:48pm UTC](https://forum.biobakery.org/t/humann-v4-output-are-different/7218/3 "2024-07-16T19:48:34Z")

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Indeed, it sounds like you pulled the HUMAnN 4 alpha source code, but we have not published the full databases for HUMAnN 4 yet, so you’d only be seeing demo-style output.

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**Author:** ![Michela\_Francesconi](https://yyz1.discourse-cdn.com/flex027/user_avatar/forum.biobakery.org/michela_francesconi/32/2874_2.png) [@Michela\_Francesconi](https://forum.biobakery.org/u/Michela_Francesconi)\
**Post date:** [July 17, 2024, 7:36am UTC](https://forum.biobakery.org/t/humann-v4-output-are-different/7218/4 "2024-07-17T07:36:12Z")

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Ok thank you! Also because when I run it I have the errore that the version vOct22 does not have the head line but I would like to use the version vJun23 (202403) for Metaphlan. How I can do? I have to wait for the new version?

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**Author:** ![franzosa](https://yyz1.discourse-cdn.com/flex027/user_avatar/forum.biobakery.org/franzosa/32/3511_2.png) [@franzosa](https://forum.biobakery.org/u/franzosa)\
**Post date:** [July 17, 2024, 7:32pm UTC](https://forum.biobakery.org/t/humann-v4-output-are-different/7218/5 "2024-07-17T19:32:31Z")

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Sorry I’m not following your question. You want to use the newest MetaPhlAn with the newest MetaPhlAn database? If so, that is discussed here:

> [@Announcing MetaPhlAn 4.1.1 release](https://forum.biobakery.org/t/announcing-metaphlan-4-1-1-release/7094):
>
> Announcement: We are pleased to share that MetaPhlAn 4.1.1 is now available, which provides fixed and consistent taxonomic identification at high taxonomic ranks, allowing accurate relative abundance estimation also at high taxonomic levels. This is enabled by a new version of the MetaPhlAn database (vJun23\_202403) containing the taxonomic updates. It is also possible to fix taxonomic profiles generated with previous versions of the MetaPhlAn database (i.e., vJun23\_202307 and earlier) by running…

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<div class="post-metadata">

**Author:** ![Michela\_Francesconi](https://yyz1.discourse-cdn.com/flex027/user_avatar/forum.biobakery.org/michela_francesconi/32/2874_2.png) [@Michela\_Francesconi](https://forum.biobakery.org/u/Michela_Francesconi)\
**Post date:** [July 18, 2024, 7:21am UTC](https://forum.biobakery.org/t/humann-v4-output-are-different/7218/6 "2024-07-18T07:21:35Z")

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No, sorry if I do not explain myself correctly. I want to use humann 3.9 with the vJun23\_202403 database version of Metaphlan but I don’t know if it is possible.
