# Humann can not run with metaphlan together

**URL:** <https://forum.biobakery.org/t/humann-can-not-run-with-metaphlan-together/774>\
**Category:** HUMAnN\
**Created:** [July 26, 2020, 1:08pm UTC](https://forum.biobakery.org/t/humann-can-not-run-with-metaphlan-together/774 "2020-07-26T13:08:33Z")\
**Posts on this page:** 1\
**Showing post:** 13

<div class="post-metadata">

**Author:** ![wenping](https://avatars.discourse-cdn.com/v4/letter/w/e56c9b/32.png) [@wenping](https://forum.biobakery.org/u/wenping)\
**Post date:** [July 30, 2020, 9:05am UTC](https://forum.biobakery.org/t/humann-can-not-run-with-metaphlan-together/774/13 "2020-07-30T09:05:04Z")

</div>

The input file is as the following:  
(py37) [zhangwenping@localhost clean\_data\_wuqi] read\_fastx.py -l 70 CRR055205\_paired.1.fastq \> /dev/null (py37) [zhangwenping@localhost clean\_data\_wuqi] more CRR055205\_paired.1.fastq  
@HWI-ST531R:260:C4GKMACXX:3:1101:1954:1955 1:N:0:GTCTG  
TAAAGTATACATTTATAATTAGTTAGGAGAAGAATTTATGTCGTTTTCAGC  
+  
CCCFFEDEHHHHGJJJIJJJJJHIJJJGGJIJIJJJJIIJHIJHIIJJIJI  
@HWI-ST531R:260:C4GKMACXX:3:1101:2131:1942 1:N:0:GTCTG  
TCANCAGTCATTATTGCTTCACCATGAACTTCAAAAAAATCACCCGTTG  
+  
@@@#4ADDHHHHHEIHEIJIIIJJJJJIJIJJIIJJIJJGBDFFHHAEH  
@HWI-ST531R:260:C4GKMACXX:3:1101:2108:1942 1:N:0:GTCTG  
CATNTTTATCATTAACTGGTCGAACAAGAATGTCAAATAATTGGGGATCGG  
+  
CCC#4ADFHHHHHJJJJJJHIJJJIJJJHIJJJJJJJJJJJJJJJIFGIIJ  
@HWI-ST531R:260:C4GKMACXX:3:1101:2487:1994 1:N:0:GTCTG  
GTCATAGATTAAGGCATGAAGAAAACTATTGTGAAGAAATTAATCTCAA  
+  
?@@DFDD?CFFFFHIIIGEHIIIIGEC@HICFDGHGEGHGHIICDG\>DH  
@HWI-ST531R:260:C4GKMACXX:3:1101:2647:1943 1:N:0:GTCTG  
AGTNGTGTCGTGTGTTGAGGGTGTTATGGAACCTGTATTTATTGACATCTG  
+  
@@?#4=BDFFFFBGFFEEGII1CCFEGEIDFFIIFDEGIICGIIFFIEFII  
@HWI-ST531R:260:C4GKMACXX:3:1101:2676:1948 1:N:0:GTCTG  
CCGNTTGGTACAATAATTCATACAGGAGATTTTAAAATAGACTATACTCCA  
+  
@@C#4ADDBFHHHJJJJJIJDHJJJJIIFHGIJIIIJEEGHIHIJIJJIJJ  
@HWI-ST531R:260:C4GKMACXX:3:1101:2694:1962 1:N:0:GTCTG  
TTTAAGACAAATTCAAGATGGGATAGAGCTATTTATACTTGGACTAGGAAG  
+  
CCCFFFFFHHHHHJJIJIJJJJIJIJJJJJJJJJIGJJJJJJJJJIJJIII  
@HWI-ST531R:260:C4GKMACXX:3:1101:2570:1982 1:N:0:GTCTG  
CTATATCATTAAATCCATCCTTATTGAGAGTTTCTATGATTGCTGCAAAAACTTC  
+  
CCCFDFDFHHFHHIIHIIIGIJIHHJHIHIAHHGIGGIJIJIJJJJJIIHJJIJJ  
(py37) [zhangwenping@localhost clean\_data\_wuqi]$

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