# Humann 3 bowtie error

**URL:** https://forum.biobakery.org/t/humann-3-bowtie-error/610
**Category:** HUMAnN
**Created:** [June 19, 2020, 12:05pm UTC](https://forum.biobakery.org/t/humann-3-bowtie-error/610 "2020-06-19T12:05:00Z")
**Posts on this page:** 1
**Showing post:** 9

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### Author: ![nasnuvens](https://avatars.discourse-cdn.com/v4/letter/n/898d66/32.png) [@nasnuvens](https://forum.biobakery.org/u/nasnuvens)
#### Post date: [May 2, 2021, 3:40pm UTC](https://forum.biobakery.org/t/humann-3-bowtie-error/610/9 "2021-05-02T15:40:35Z")

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I’m also having this same error message, but only when I include in my database long sequences (\> 200000 bp). You can find attached a file which I got an error message after using the command:

`humann --input seqError2.fasta.txt --output /vol/volume2/outputTest`

However, I have no error message if the long sequences is removed from the file, or if it is put at the beginning of the file (it is a bit strange that I can solve the problem by changing the position of the long sequence).

[seqError2.fasta.txt](https://forum.biobakery.org/uploads/short-url/yLIPqkOyW6jJE7Fdhw9hgFxAHqt.txt) (2.5 MB)

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