# Humann 3.9 Demo file results

**URL:** <https://forum.biobakery.org/t/humann-3-9-demo-file-results/6992>\
**Category:** HUMAnN\
**Created:** [May 2, 2024, 1:48pm UTC](https://forum.biobakery.org/t/humann-3-9-demo-file-results/6992 "2024-05-02T13:48:22Z")\
**Posts on this page:** 1\
**Page:** 1

<div class="post-metadata">

**Author:** ![Yossi\_Cohen](https://yyz1.discourse-cdn.com/flex027/user_avatar/forum.biobakery.org/yossi_cohen/32/2842_2.png) [@Yossi\_Cohen](https://forum.biobakery.org/u/Yossi_Cohen)\
**Post date:** [May 2, 2024, 1:48pm UTC](https://forum.biobakery.org/t/humann-3-9-demo-file-results/6992/1 "2024-05-02T13:48:22Z")

</div>

Hi,  
I’m using Humann 3.9 coupled with metaphlan 4.1.  
I’ve downloaded the demo.fastq.gz + the demo databases.

When i run the demo file i receive different results than what is mentioned in the Humann tutorial.

My run looks like this:

````auto
Running metaphlan ........

Found g __GGB1511.s__ GGB1511_SGB2091 : 81.49% of mapped reads ( )
Found t__SGB1871 : 16.03% of mapped reads ( s__Bacteroides_ovatus,g __Bacteroides.s__ Bacteroides_sp_2_2_4,g __Bacteroides.s__ Bacteroides_sp_AM56_10ce,g __Bacteroides.s__ Bacteroides_sp_AF32_8BH,g __Bacteroides.s__ Bacteroides_sp_KFT8,g __Bacteroides.s__ Bacteroides_sp_HMSC073E02,g __Bacteroides.s__ Bacteroides_sp_3_1_23,g __Bacteroides.s__ Bacteroides_kribbi,g __Bacteroides.s__ Bacteroides_sp_OF03_11BH,g __Bacteroides.s__ Bacteroides_sp_MSK_17_76,g __Bacteroides.s__ Bacteroides_sp_MSK_18_37 )
Found t__SGB2301 : 2.03% of mapped reads ( s__Alistipes_finegoldii,g __Alistipes.s__ Alistipes_sp_HGB5 )
Found t__SGB1814 : 0.45% of mapped reads ( s__Bacteroides_vulgatus,g __Phocaeicola.s__ Phocaeicola_dorei )
Total species selected from prescreen: 19
Selected species explain 100.00% of predicted community composition
Creating custom ChocoPhlAn database ........
Running bowtie2-build ........
Running bowtie2 ........
Total bugs from nucleotide alignment: 1
g __Bacteroides.s__ Bacteroides_vulgatus: 25357 hits
Total gene families from nucleotide alignment: 838
Unaligned reads after nucleotide alignment: 82.3496655367 %
Running diamond ........
Aligning to reference database: uniref90_demo_prots_v201901b.dmnd
Total bugs after translated alignment: 1
g __Bacteroides.s__ Bacteroides_vulgatus: 25357 hits
Total gene families after translated alignment: 838
Unaligned reads after translated alignment: 82.3496655367 %
Computing gene families ...
Computing pathways abundance and coverage ...

Output files created: 
/home/ec2-user/humann3_test/demo3_fastq/demo3_genefamilies.tsv
/home/ec2-user/humann3_test/demo3_fastq/demo3_pathabundance.tsv
/home/ec2-user/humann3_test/demo3_fastq/demo3_pathcoverage.tsv```
 

Can you share whether this output is OK since it does not match what appears in the toturial. 

Thanks
````