# How to install the latest metaphlan reference DB? **URL:** \ **Category:** MetaPhlAn\ **Created:** [May 2, 2022, 1:52pm UTC](https://forum.biobakery.org/t/how-to-install-the-latest-metaphlan-reference-db/3522 "2022-05-02T13:52:55Z")\ **Posts on this page:** 17\ **Page:** 1
**Author:** ![shengxinf](https://yyz1.discourse-cdn.com/flex027/user_avatar/forum.biobakery.org/shengxinf/32/1421_2.png) [@shengxinf](https://forum.biobakery.org/u/shengxinf)\ **Post date:** [May 2, 2022, 1:52pm UTC](https://forum.biobakery.org/t/how-to-install-the-latest-metaphlan-reference-db/3522/1 "2022-05-02T13:52:55Z")
Hi there: The metaphlan software is very cool, which is very helpful to me. Here，I have a question about the ‘mpa\_vJan21\_CHOCOPhlAnSGB\_202103’ installation. After I downloaded the ‘mpa\_vjan21\_chocophlansGB\_202103.tar’ to the specified location, useing the github command " `metaphlan --install --index mpa_vJan21_CHOCOPhlAnSGB_202103 -- Bowtie2db ` " to build the Metaphlan database.But it does not work: ![metaphlan3-error](https://canada1.discourse-cdn.com/flex027/uploads/biobakery/original/2X/f/ffc45b0141d99082420a99967cbd06bc27963798.png) The installation address of the Metaphlan database is as follows: ![metaphlan-DB](https://canada1.discourse-cdn.com/flex027/uploads/biobakery/original/2X/c/cfc14e4933e110d859f86fdba5ce2e0cbab64cec.png) So I delete ‘mpa\_vjan21\_chocophlansGB\_202103.fna’ ,and run the `metaphlan --install` command again: ![metaphlan --install](https://canada1.discourse-cdn.com/flex027/uploads/biobakery/original/2X/a/a634647101ed91150dea823f858e68be271f72a8.png) But metaphlan does not generate the .bt2 file(like mpa\_v31\_CHOCOPhlAn\_201901.1.bt2)，so how to build the mpa\_vJan21\_CHOCOPhlAnSGB\_202103 (the latest metaphlan reference DB) ? Any help would be appreciated! Thanks! ---
**Author:** ![mherold1](https://yyz1.discourse-cdn.com/flex027/user_avatar/forum.biobakery.org/mherold1/32/1431_2.png) [@mherold1](https://forum.biobakery.org/u/mherold1)\ **Post date:** [May 4, 2022, 3:20pm UTC](https://forum.biobakery.org/t/how-to-install-the-latest-metaphlan-reference-db/3522/2 "2022-05-04T15:20:03Z")
Why did it create mpa\_v31\_CHOCOPhlAn\_201901.1.bt2 for you? For me it is still installing mpa\_v30\_CHOCOPhlAn\_201901 because that is set in: [http://cmprod1.cibio.unitn.it/biobakery3/metaphlan\_databases/mpa\_latest](http://cmprod1.cibio.unitn.it/biobakery3/metaphlan_databases/mpa_latest) The version that you mentioned I found here: [http://cmprod1.cibio.unitn.it/biobakery4/metaphlan\_databases/](http://cmprod1.cibio.unitn.it/biobakery4/metaphlan_databases/) ---
**Author:** ![aitor.blancomiguez](https://yyz1.discourse-cdn.com/flex027/user_avatar/forum.biobakery.org/aitor.blancomiguez/32/86_2.png) [@aitor.blancomiguez](https://forum.biobakery.org/u/aitor.blancomiguez)\ **Post date:** [May 18, 2022, 2:11pm UTC](https://forum.biobakery.org/t/how-to-install-the-latest-metaphlan-reference-db/3522/3 "2022-05-18T14:11:23Z")
Hi @shengxinf , Thanks for getting in touch. The code to use the mpa\_vJan21 version of the MetaPhlAn database (i.e MetaPhlAn 4) is not available yet, we hope to release the code in the following weeks. In the meanwhile, the last stable version of MetaPhlAn is mpa\_v3.0. For a correct installation of this version, you should just run `metaphlan --install` ---
**Author:** ![imontero](https://yyz1.discourse-cdn.com/flex027/user_avatar/forum.biobakery.org/imontero/32/3411_2.png) [@imontero](https://forum.biobakery.org/u/imontero)\ **Post date:** [September 26, 2022, 7:25am UTC](https://forum.biobakery.org/t/how-to-install-the-latest-metaphlan-reference-db/3522/4 "2022-09-26T07:25:40Z")
I have instaled in a conda environment MetaPhlAn version 4.0.2 (22 Sep 2022) and humann v3.5. I try to run the code > humann --input demo.fastq.gz --output demo\_fastq --threads 4 but I get the same error “No MetaPhlAn BowTie2 database found (–index option)!” with diferent metaphlan databases: mpa\_v30\_CHOCOPhlAn\_201901 mpa\_v31\_CHOCOPhlAn\_201901 In case of mpa\_vJan21\_CHOCOPhlAnSGB\_202103 metaphlan --install create bt2l.tmp files instead of bt2. > ---
**Author:** ![imontero](https://yyz1.discourse-cdn.com/flex027/user_avatar/forum.biobakery.org/imontero/32/3411_2.png) [@imontero](https://forum.biobakery.org/u/imontero)\ **Post date:** [September 26, 2022, 7:54am UTC](https://forum.biobakery.org/t/how-to-install-the-latest-metaphlan-reference-db/3522/5 "2022-09-26T07:54:05Z")
If I downgrade to metaphlan 3.1 and humann 3.1 problems disapeared ---
**Author:** ![aitor.blancomiguez](https://yyz1.discourse-cdn.com/flex027/user_avatar/forum.biobakery.org/aitor.blancomiguez/32/86_2.png) [@aitor.blancomiguez](https://forum.biobakery.org/u/aitor.blancomiguez)\ **Post date:** [September 27, 2022, 7:44am UTC](https://forum.biobakery.org/t/how-to-install-the-latest-metaphlan-reference-db/3522/6 "2022-09-27T07:44:58Z")
Hi @imontero The bowtie2 database for mpa\_vJan21 is a large database, so the output format is bt2l instead of bt2. If you were still seeing the _.tmp files, it might mean that you didn’t finish building the database correctly. My suggestion would be to remove the mpa\_vJan21\_CHOCOPhlAnSGB\_202103_ files and try to redownload the db again with metaphlan `--force_download` ---
**Author:** ![imontero](https://yyz1.discourse-cdn.com/flex027/user_avatar/forum.biobakery.org/imontero/32/3411_2.png) [@imontero](https://forum.biobakery.org/u/imontero)\ **Post date:** [September 27, 2022, 5:34pm UTC](https://forum.biobakery.org/t/how-to-install-the-latest-metaphlan-reference-db/3522/7 "2022-09-27T17:34:59Z")
metaphlan --install --force\_download --bowtie2db ./ --nproc 4 Downloading [http://cmprod1.cibio.unitn.it/biobakery4/metaphlan\_databases/mpa\_latest](http://cmprod1.cibio.unitn.it/biobakery4/metaphlan_databases/mpa_latest) Downloading file of size: 0.00 MB 0.01 MB 25600.00 % 130.03 MB/sec 0 min -0 sec Downloading MetaPhlAn database Please note due to the size this might take a few minutes Downloading [http://cmprod1.cibio.unitn.it/biobakery4/metaphlan\_databases/mpa\_vJan21\_CHOCOPhlAnSGB\_202103.tar](http://cmprod1.cibio.unitn.it/biobakery4/metaphlan_databases/mpa_vJan21_CHOCOPhlAnSGB_202103.tar) Downloading file of size: 2623.07 MB 2623.07 MB 100.00 % 12.46 MB/sec 0 min -0 sec Downloading [http://cmprod1.cibio.unitn.it/biobakery4/metaphlan\_databases/mpa\_vJan21\_CHOCOPhlAnSGB\_202103.md5](http://cmprod1.cibio.unitn.it/biobakery4/metaphlan_databases/mpa_vJan21_CHOCOPhlAnSGB_202103.md5) Downloading file of size: 0.00 MB 0.01 MB 11702.86 % 64.38 MB/sec 0 min -0 sec Decompressing ./mpa\_vJan21\_CHOCOPhlAnSGB\_202103\_SGB.fna.bz2 into ./mpa\_vJan21\_CHOCOPhlAnSGB\_202103\_SGB.fna Decompressing ./mpa\_vJan21\_CHOCOPhlAnSGB\_202103\_VSG.fna.bz2 into ./mpa\_vJan21\_CHOCOPhlAnSGB\_202103\_VSG.fna Joining FASTA databases Building Bowtie2 indexes Fatal error running ‘bowtie2-build --quiet --threads 4 -f ./mpa\_vJan21\_CHOCOPhlAnSGB\_202103.fna ./mpa\_vJan21\_CHOCOPhlAnSGB\_202103’ Error message: ‘Command ‘[‘bowtie2-build’, ‘–quiet’, ‘–threads’, ‘4’, ‘-f’, ‘./mpa\_vJan21\_CHOCOPhlAnSGB\_202103.fna’, ‘./mpa\_vJan21\_CHOCOPhlAnSGB\_202103’]’ returned non-zero exit status 247.’ ---
**Author:** ![aitor.blancomiguez](https://yyz1.discourse-cdn.com/flex027/user_avatar/forum.biobakery.org/aitor.blancomiguez/32/86_2.png) [@aitor.blancomiguez](https://forum.biobakery.org/u/aitor.blancomiguez)\ **Post date:** [September 30, 2022, 8:07am UTC](https://forum.biobakery.org/t/how-to-install-the-latest-metaphlan-reference-db/3522/8 "2022-09-30T08:07:58Z")
Hi @imontero By your error, it seems you ran out of resources (probably RAM) when generating the indexes. MetaPhlAn 4 database significantly increased with respect to version 3 and typically requires around 16-20GB or RAM ---
**Author:** ![imontero](https://yyz1.discourse-cdn.com/flex027/user_avatar/forum.biobakery.org/imontero/32/3411_2.png) [@imontero](https://forum.biobakery.org/u/imontero)\ **Post date:** [September 30, 2022, 8:24am UTC](https://forum.biobakery.org/t/how-to-install-the-latest-metaphlan-reference-db/3522/9 "2022-09-30T08:24:23Z")
Thank you for your advice. It is strage because my PC has 32Gb of RAM, however I will upgrade my PC to 64GB soon. I will try them. ---
**Author:** ![Bernhard](https://avatars.discourse-cdn.com/v4/letter/b/c37758/32.png) [@Bernhard](https://forum.biobakery.org/u/Bernhard)\ **Post date:** [October 24, 2022, 1:58pm UTC](https://forum.biobakery.org/t/how-to-install-the-latest-metaphlan-reference-db/3522/10 "2022-10-24T13:58:26Z")
I also ran into the same sudden error when executing bowtie2-build for MetaPhlAn4 with the process exit status 247. I had around 28 GB of RAM available for a virtual machine, though suddenly the process terminated. Bowtie2 has not finished and left me with the following files: ```bash (base) bernhard@system metaphlan_db % ls -lah total 109544408 drwxr-xr-x 30 bernhard staff 960B 24 Okt 13:03 . drwxr-xr-x@ 10 bernhard staff 320B 22 Okt 22:05 .. -rw-r--r--@ 1 bernhard staff 10K 22 Okt 23:44 .DS_Store -rw-r--r-- 1 bernhard staff 32B 22 Okt 22:06 mpa_latest -rw-r--r-- 1 bernhard staff 4,1G 24 Okt 04:45 mpa_vJan21_CHOCOPhlAnSGB_202103.1.bt2l.tmp -rw-r--r-- 1 bernhard staff 4,4G 24 Okt 04:32 mpa_vJan21_CHOCOPhlAnSGB_202103.2.bt2l.tmp -rw-r--r-- 1 bernhard staff 85M 23 Okt 16:46 mpa_vJan21_CHOCOPhlAnSGB_202103.3.bt2l.tmp -rw-r--r-- 1 bernhard staff 2,2G 23 Okt 16:46 mpa_vJan21_CHOCOPhlAnSGB_202103.4.bt2l.tmp -rw-r--r-- 1 bernhard staff 10G 23 Okt 13:07 mpa_vJan21_CHOCOPhlAnSGB_202103.fna -rw-r--r-- 1 bernhard staff 70B 22 Okt 22:22 mpa_vJan21_CHOCOPhlAnSGB_202103.md5 -rw-rw-r-- 1 bernhard staff 53M 1 Apr 2022 mpa_vJan21_CHOCOPhlAnSGB_202103.pkl -rw-r--r-- 1 bernhard staff 1,7G 24 Okt 13:06 mpa_vJan21_CHOCOPhlAnSGB_202103.rev.1.bt2l.tmp -rw-r--r-- 1 bernhard staff 2,3G 24 Okt 13:06 mpa_vJan21_CHOCOPhlAnSGB_202103.rev.2.bt2l.tmp -rw-r--r-- 1 bernhard staff 2,2G 24 Okt 11:33 mpa_vJan21_CHOCOPhlAnSGB_202103.rev.24.sa -rw-r--r-- 1 bernhard staff 2,2G 24 Okt 12:22 mpa_vJan21_CHOCOPhlAnSGB_202103.rev.25.sa -rw-r--r-- 1 bernhard staff 1,8G 24 Okt 12:26 mpa_vJan21_CHOCOPhlAnSGB_202103.rev.26.sa -rw-r--r-- 1 bernhard staff 1,7G 24 Okt 12:38 mpa_vJan21_CHOCOPhlAnSGB_202103.rev.27.sa -rw-r--r-- 1 bernhard staff 2,0G 24 Okt 13:01 mpa_vJan21_CHOCOPhlAnSGB_202103.rev.28.sa -rw-r--r-- 1 bernhard staff 1,4G 24 Okt 12:40 mpa_vJan21_CHOCOPhlAnSGB_202103.rev.29.sa -rw-r--r-- 1 bernhard staff 1,0G 24 Okt 13:06 mpa_vJan21_CHOCOPhlAnSGB_202103.rev.30.sa -rw-r--r-- 1 bernhard staff 918M 24 Okt 13:05 mpa_vJan21_CHOCOPhlAnSGB_202103.rev.31.sa -rw-r--r-- 1 bernhard staff 667M 24 Okt 13:06 mpa_vJan21_CHOCOPhlAnSGB_202103.rev.32.sa -rw-r--r-- 1 bernhard staff 568M 24 Okt 13:06 mpa_vJan21_CHOCOPhlAnSGB_202103.rev.33.sa -rw-r--r-- 1 bernhard staff 133M 24 Okt 13:06 mpa_vJan21_CHOCOPhlAnSGB_202103.rev.34.sa -rw-r--r--@ 1 bernhard staff 2,6G 22 Okt 22:22 mpa_vJan21_CHOCOPhlAnSGB_202103.tar -rw-r--r-- 1 bernhard staff 9,2G 23 Okt 11:33 mpa_vJan21_CHOCOPhlAnSGB_202103_SGB.fna -rw-rw-r-- 1 bernhard staff 43K 11 Jun 2021 mpa_vJan21_CHOCOPhlAnSGB_202103_VINFO.csv -rw-r--r-- 1 bernhard staff 841M 23 Okt 10:39 mpa_vJan21_CHOCOPhlAnSGB_202103_VSG.fna -rw-r--r--@ 1 bernhard staff 29M 22 Aug 16:51 mpa_vJan21_CHOCOPhlAnSGB_202103_marker_info.txt.bz2 -rw-r--r--@ 1 bernhard staff 380K 25 Aug 10:25 mpa_vJan21_CHOCOPhlAnSGB_202103_species.txt.bz2 ``` I guess it is better to stick with the older MetaPhlAn3 version then or move to a system with more RAM. Best regards, Bernhard ---
**Author:** ![timyerg](https://yyz1.discourse-cdn.com/flex027/user_avatar/forum.biobakery.org/timyerg/32/959_2.png) [@timyerg](https://forum.biobakery.org/u/timyerg)\ **Post date:** [November 17, 2022, 7:37am UTC](https://forum.biobakery.org/t/how-to-install-the-latest-metaphlan-reference-db/3522/11 "2022-11-17T07:37:00Z")
Had the same issue. 32 Gb on the remote cluster was not enough. Allocated 64 GB and it worked (probably you don’t need 64 😀 ). ---
**Author:** ![aitor.blancomiguez](https://yyz1.discourse-cdn.com/flex027/user_avatar/forum.biobakery.org/aitor.blancomiguez/32/86_2.png) [@aitor.blancomiguez](https://forum.biobakery.org/u/aitor.blancomiguez)\ **Post date:** [November 21, 2022, 1:03pm UTC](https://forum.biobakery.org/t/how-to-install-the-latest-metaphlan-reference-db/3522/12 "2022-11-21T13:03:26Z")
Yes, building the new MetaPhlAn 4 database requires a great amount of RAM. We are currently working on making the pre-build database available to download to avoid this inconvenience. We will keep you posted ---
**Author:** ![aitor.blancomiguez](https://yyz1.discourse-cdn.com/flex027/user_avatar/forum.biobakery.org/aitor.blancomiguez/32/86_2.png) [@aitor.blancomiguez](https://forum.biobakery.org/u/aitor.blancomiguez)\ **Post date:** [November 25, 2022, 8:33am UTC](https://forum.biobakery.org/t/how-to-install-the-latest-metaphlan-reference-db/3522/13 "2022-11-25T08:33:29Z")
I uploaded a precomputed version of the bt2 database here: [http://cmprod1.cibio.unitn.it/biobakery4/metaphlan\_databases/bowtie2\_indexes/mpa\_vJan21\_CHOCOPhlAnSGB\_202103\_bt2.tar](http://cmprod1.cibio.unitn.it/biobakery4/metaphlan_databases/bowtie2_indexes/mpa_vJan21_CHOCOPhlAnSGB_202103_bt2.tar) Let me know if you have any problems with it ---
**Author:** ![rda](https://avatars.discourse-cdn.com/v4/letter/r/c2a13f/32.png) [@rda](https://forum.biobakery.org/u/rda)\ **Post date:** [March 22, 2023, 10:04pm UTC](https://forum.biobakery.org/t/how-to-install-the-latest-metaphlan-reference-db/3522/14 "2023-03-22T22:04:38Z")
```auto $ humann --version humann v3.6.1 $ metaphlan --version MetaPhlAn version 4.0.6 (1 Mar 2023) $ biobakery_workflows --version biobakery_workflows v3.1 ``` When running: ```auto humann --input /u/home/staff1/cusgunx/ATS-TEST/biobakery/output_data3/kneaddata/main/HD32R1_subsample.fastq.gz --output /u/home/staff1/cusgunx/ATS-TEST/biobakery/output_data3/humann/main --o-log /u/home/staff1/cusgunx/ATS-TEST/biobakery/output_data3/humann/main/HD32R1_subsample.log --threads 1 --taxonomic-profile /u/home/staff1/cusgunx/ATS-TEST/biobakery/output_data3/metaphlan/main/HD32R1_subsample_taxonomic_profile.tsv ``` I get: ```auto ERROR: The MetaPhlAn taxonomic profile provided was not generated with the database version v3 or vJan21 . Please update your version of MetaPhlAn to at least v3.0 or if you are using MetaPhlAn v4 please use the database vJan21. ``` Indeed when downloading the metaphlan DB with: `metaphlan --install` the db installed is: vOct22 this is a consequence of: [http://cmprod1.cibio.unitn.it/biobakery4/metaphlan\_databases/mpa\_latest](http://cmprod1.cibio.unitn.it/biobakery4/metaphlan_databases/mpa_latest) containing: ```auto mpa_vOct22_CHOCOPhlAnSGB_202212 ``` I downloaded the precomputed version of the bt2 database from: [http://cmprod1.cibio.unitn.it/biobakery4/metaphlan\_databases/bowtie2\_indexes/mpa\_vJan21\_CHOCOPhlAnSGB\_202103\_bt2.tar](http://cmprod1.cibio.unitn.it/biobakery4/metaphlan_databases/bowtie2_indexes/mpa_vJan21_CHOCOPhlAnSGB_202103_bt2.tar) (as suggested in [How to install the latest metaphlan reference DB? - #13 by aitor.blancomiguez](https://forum.biobakery.org/t/how-to-install-the-latest-metaphlan-reference-db/3522/13) and I untarred the file and placed the bt2l files in the methaplan\_databases directory but even after doing so I still get the same error. Possibly because I miss a pkl and a csv file for the vJan21? **Question 1** : is there a way to force a specific version to be downloaded and computed? **Question 2** : what is the command to perform the computation locally after downloading the files from [Index of /biobakery4/metaphlan\_databases](http://cmprod1.cibio.unitn.it/biobakery4/metaphlan_databases/) for vJan21? **Question 3** : I see that vJan21 is hardcoded in many files humann and metaphlan (e.g., humann/config.py, humann-3.6.1-py3.9.egg-info/SOURCES.txt, MetaPhlAn-4.0.6.dist-info/METADATA, etc.), shouldn’t accordingly mpa\_latest in [Index of /biobakery4/metaphlan\_databases](http://cmprod1.cibio.unitn.it/biobakery4/metaphlan_databases/) point to vJan21 and not vOct22 or the version be updated in humann and metaphlan? Thanks, RD ---
**Author:** ![rda](https://avatars.discourse-cdn.com/v4/letter/r/c2a13f/32.png) [@rda](https://forum.biobakery.org/u/rda)\ **Post date:** [March 22, 2023, 10:19pm UTC](https://forum.biobakery.org/t/how-to-install-the-latest-metaphlan-reference-db/3522/15 "2023-03-22T22:19:12Z")
OK, I see that the answer to my Queston 1 in [How to install the latest metaphlan reference DB? - #14 by rda](https://forum.biobakery.org/t/how-to-install-the-latest-metaphlan-reference-db/3522/14) is provided in: > **[MetaPhlAn 4](https://github.com/biobakery/MetaPhlAn/wiki/MetaPhlAn-4)** > > MetaPhlAn is a computational tool for profiling the composition of microbial communities from metagenomic shotgun sequencing data - biobakery/MetaPhlAn > By default, the latest MetaPhlAn database is downloaded and built. You can download a specific version with the `--index` parameter > > ```auto > $ metaphlan --install --index mpa_vJan21_CHOCOPhlAnSGB_202103 --bowtie2db > > ``` ---
**Author:** ![rda](https://avatars.discourse-cdn.com/v4/letter/r/c2a13f/32.png) [@rda](https://forum.biobakery.org/u/rda)\ **Post date:** [March 23, 2023, 12:34am UTC](https://forum.biobakery.org/t/how-to-install-the-latest-metaphlan-reference-db/3522/16 "2023-03-23T00:34:30Z")
Update to: [How to install the latest metaphlan reference DB? - #15 by rda](https://forum.biobakery.org/t/how-to-install-the-latest-metaphlan-reference-db/3522/15) Even after downloading the DB with the --index, the step: ```auto $ humann --input /u/home/staff1/cusgunx/ATS-TEST/biobakery/output_data4/kneaddata/main/HD32R1_subsample.fastq.gz --output /u/home/staff1/cusgunx/ATS-TEST/biobakery/output_data4/humann/main --o-log /u/home/staff1/cusgunx/ATS-TEST/biobakery/output_data4/humann/main/HD32R1_subsample.log --threads 1 --taxonomic-profile /u/home/staff1/cusgunx/ATS-TEST/biobakery/output_data4/metaphlan/main/HD32R1_subsample_taxonomic_profile.tsv ``` still fails with error: ```auto ERROR: The MetaPhlAn taxonomic profile provided was not generated with the database version v3 or vJan21 . Please update your version of MetaPhlAn to at least v3.0 or if you are using MetaPhlAn v4 please use the database vJan21. ``` I think is caused by the fact that when computing the taxonomic profile metaphlan chooses the version of the DB as declared in mpa\_latest, I am not sure how to correct this step (especially when it occurs from within biobakery\_workflow). Please see below the first few lines of the tsv file: ```auto $ head /u/home/staff1/cusgunx/ATS-TEST/biobakery/output_data4/metaphlan/main/HD32R1_subsample_taxonomic_profile.tsv #mpa_vOct22_CHOCOPhlAnSGB_202212 #/u/local/apps/PYTHON-VIRT-ENVS/3.9.6/biobakery/bin/metaphlan /u/home/staff1/cusgunx/ATS-TEST/biobakery/output_data4/kneaddata/main/HD32R1_subsample.fastq.gz --input_type fastq --output_file /u/home/staff1/cusgunx/ATS-TEST/biobakery/output_data4/metaphlan/main/HD32R1_subsample_taxonomic_profile.tsv --samout /u/home/staff1/cusgunx/ATS-TEST/biobakery/output_data4/metaphlan/main/HD32R1_subsample_bowtie2.sam --nproc 1 --no_map --tmp_dir /u/home/staff1/cusgunx/ATS-TEST/biobakery/output_data4/metaphlan/main #34596 reads processed #SampleID Metaphlan_Analysis #clade_name NCBI_tax_id relative_abundance additional_species k__Bacteria 2 100.0 k __Bacteria|p__ Bacteroidetes 2|976 76.7975 k __Bacteria|p__ Firmicutes 2|1239 18.81751 k __Bacteria|p__ Actinobacteria 2|201174 4.38499 k __Bacteria|p__ Bacteroidetes|c__Bacteroidia 2|976|200643 76.7975 ``` Any suggestion would be appreciated. Thanks ---
**Author:** ![aitor.blancomiguez](https://yyz1.discourse-cdn.com/flex027/user_avatar/forum.biobakery.org/aitor.blancomiguez/32/86_2.png) [@aitor.blancomiguez](https://forum.biobakery.org/u/aitor.blancomiguez)\ **Post date:** [March 23, 2023, 9:14am UTC](https://forum.biobakery.org/t/how-to-install-the-latest-metaphlan-reference-db/3522/17 "2023-03-23T09:14:34Z")
As the installation is done via humann, I think if you move the question to the humann subforum ([HUMAnN - The bioBakery help forum](https://forum.biobakery.org/c/microbial-community-profiling/humann/5)) they will better address your question