# How to create Annotation file

**URL:** <https://forum.biobakery.org/t/how-to-create-annotation-file/626>\
**Category:** GraPhlAn\
**Created:** [June 23, 2020, 9:04am UTC](https://forum.biobakery.org/t/how-to-create-annotation-file/626 "2020-06-23T09:04:12Z")\
**Posts on this page:** 3\
**Page:** 1

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**Author:** ![Suwitchaya](https://yyz1.discourse-cdn.com/flex027/user_avatar/forum.biobakery.org/suwitchaya/32/266_2.png) [@Suwitchaya](https://forum.biobakery.org/u/Suwitchaya)\
**Post date:** [June 23, 2020, 9:04am UTC](https://forum.biobakery.org/t/how-to-create-annotation-file/626/1 "2020-06-23T09:04:12Z")

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Now, I analyte gut microbiome by using qiime2 pipeline.

So, I interest to present data in circular representations of taxonomic and phylogenetic trees by using GraPhlAn but I have no idea how to create annotation file like guide\_rings.txt in Tutorial [https://github.com/biobakery/biobakery/wiki/graphlan](https://github.com/biobakery/biobakery/wiki/graphlan)

Kind Regards,  
Suwitchaya

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**Author:** ![aaron\_walsh](https://yyz1.discourse-cdn.com/flex027/user_avatar/forum.biobakery.org/aaron_walsh/32/554_2.png) [@aaron\_walsh](https://forum.biobakery.org/u/aaron_walsh)\
**Post date:** [January 13, 2021, 2:27pm UTC](https://forum.biobakery.org/t/how-to-create-annotation-file/626/2 "2021-01-13T14:27:29Z")

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Hi Suwitchaya,

Thanks for your interest in GraPhlAn. To make a tree with GraPhlAn, you need two files: a tree file (e.g. [guide.txt](https://github.com/biobakery/graphlan/blob/master/examples/guide/guide.txt)) and an annotation file (e.g. [guide\_rings.txt](https://github.com/biobakery/graphlan/releases/download/1.1.3/guide_rings.txt)). The tree file lists all of your taxa. Here, each taxonomic rank is separated by a “.” (e.g. Family.Genus.Species), and each species will be plotted as a leaf on the tree. The annotation file is a tab delimited file that specifies the parameters of the image, thereby allowing you to add information to the tree. The GraPhlAn GitHub ([https://github.com/biobakery/graphlan](https://github.com/biobakery/graphlan)) page provides information on all of the ways in which you can annotate your tree (e.g. you can change the colour of leaves, you can add rings, etc.). What sort of annotation that you want to add to your plot? Thanks again.

Regards,  
Aaron.

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**Author:** ![meslinmanea](https://yyz1.discourse-cdn.com/flex027/user_avatar/forum.biobakery.org/meslinmanea/32/1107_2.png) [@meslinmanea](https://forum.biobakery.org/u/meslinmanea)\
**Post date:** [November 10, 2021, 1:53pm UTC](https://forum.biobakery.org/t/how-to-create-annotation-file/626/3 "2021-11-10T13:53:15Z")

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Hi,

I’m trying to create an annotation file for my newick tree and I wanted to know if there is a tool or if you had a tip to create it quickly and efficiently for many branches and many annotations to add. Thank you for your attention.