Thanks for your help Andrew, we coded the age as a discrete variable to use LEfSe first, we kept that in MaAsLin… then by inertia it got dragged as discrete into MMUPHin. I went back to the original numbers and the analysis run fine. Thanks for your help.
The package is great, maybe it is planned for future iterations like it was commented for MaAsLin but a follow up question or request would be to ask if there is a way to manually adjust the size of the ‘feature label’ in the forest .pdf plot, with pathways or broadly speaking HUMAnN data the large name is abbreviated since it can’t fit the page.