# Error with the examples files

**URL:** <https://forum.biobakery.org/t/error-with-the-examples-files/7754>\
**Category:** HUMAnN\
**Created:** [January 16, 2025, 10:14am UTC](https://forum.biobakery.org/t/error-with-the-examples-files/7754 "2025-01-16T10:14:12Z")\
**Posts on this page:** 2\
**Page:** 1

<div class="post-metadata">

**Author:** ![Michela\_Francesconi](https://yyz1.discourse-cdn.com/flex027/user_avatar/forum.biobakery.org/michela_francesconi/32/2874_2.png) [@Michela\_Francesconi](https://forum.biobakery.org/u/Michela_Francesconi)\
**Post date:** [January 16, 2025, 10:14am UTC](https://forum.biobakery.org/t/error-with-the-examples-files/7754/1 "2025-01-16T10:14:12Z")

</div>

Hello,  
I’m having some problems with the examples files. I want to run HuManN (v4.0)

```auto
humann --input "$fastq_file" --output "$sample_output_dir" 

```

But I always have this error

```auto
humann.search.prescreen - ERROR: The relative abundance and coverage were not found in the MetaPhlAn taxonomic profile. Please run MetaPhlAn with the option(s): -t rel_ab_w_read_stats.

```

So I tried with:

```auto
humann --input "$fastq_file" --output "$sample_output_dir" --metaphlan-options="-t rel_ab_w_read_stats"

humann --input "$fastq_file" --output "$sample_output_dir" --metaphlan-options="-t rel_ab"

humann --input "$fastq_file" --output "$sample_output_dir" --metaphlan-options="-t reads_map"

```

But I always have the same error, and nothing changes. Do you have any idea? Because I have `metaphlan --version MetaPhlAn version 4.0.0 (22 Aug 2022)`  
and if I run it alone, I do not have any errors!

Thank you

---

<div class="post-metadata">

**Author:** ![franzosa](https://yyz1.discourse-cdn.com/flex027/user_avatar/forum.biobakery.org/franzosa/32/3511_2.png) [@franzosa](https://forum.biobakery.org/u/franzosa)\
**Post date:** [February 13, 2025, 10:36pm UTC](https://forum.biobakery.org/t/error-with-the-examples-files/7754/2 "2025-02-13T22:36:33Z")

</div>

Sorry for the slow reply. Something about this is strange since HUMAnN should automatically be calling MetaPhlAn and asking for the extra read stats. This sort of error would occur if (e.g.) you ran MetaPhlAn outside of HUMAnN without asking for read stats and then tried to feed the resulting MetaPhlAn output to HUMAnN. I’m assuming that is not the case here?

It sounds like HUMAnN finishes running MetaPhlAn before it hits this error. Are you able to share the MetaPhlAn output here?