# Error uploading data for lefse

**URL:** <https://forum.biobakery.org/t/error-uploading-data-for-lefse/1089>\
**Category:** LEfSe\
**Created:** [September 29, 2020, 11:16am UTC](https://forum.biobakery.org/t/error-uploading-data-for-lefse/1089 "2020-09-29T11:16:19Z")\
**Posts on this page:** 4\
**Page:** 1

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**Author:** ![stephen.moore](https://avatars.discourse-cdn.com/v4/letter/s/a87d85/32.png) [@stephen.moore](https://forum.biobakery.org/u/stephen.moore)\
**Post date:** [September 29, 2020, 11:16am UTC](https://forum.biobakery.org/t/error-uploading-data-for-lefse/1089/1 "2020-09-29T11:16:19Z")

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Hi, I have attempted to upload data to Galaxy/Hutlab for lefse analysis but I am getting an error. The error is “ **Failed:** Bad Request (400)”. I have tried using the data provided by the lefse tutorial (hmp\_small\_aerobiosis.txt) and my own data, and I receive the same error each time. I have successfully uploaded these datasets for lefse analysis on previous occasions, but I have been receiving the upload error since yesterday (29/09/20).  
Hopefully someone can help?

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**Author:** ![Kelsey\_Thompson](https://yyz1.discourse-cdn.com/flex027/user_avatar/forum.biobakery.org/kelsey_thompson/32/65_2.png) [@Kelsey\_Thompson](https://forum.biobakery.org/u/Kelsey_Thompson)\
**Post date:** [October 2, 2020, 11:03am UTC](https://forum.biobakery.org/t/error-uploading-data-for-lefse/1089/2 "2020-10-02T11:03:22Z")

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Hi Stephen,

Sorry that you had trouble connecting to the server! This is an intermittent issue that we are aware of and have been working to find a permanent fix for. Can you try again and see if you get the same issue?

Best,  
Kelsey

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**Author:** ![stephen.moore](https://avatars.discourse-cdn.com/v4/letter/s/a87d85/32.png) [@stephen.moore](https://forum.biobakery.org/u/stephen.moore)\
**Post date:** [October 2, 2020, 11:15am UTC](https://forum.biobakery.org/t/error-uploading-data-for-lefse/1089/3 "2020-10-02T11:15:36Z")

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Hi Kelsey,  
It is working again. Thank you!  
Regards,  
Stephen.

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**Author:** ![Heleen\_Delbeke](https://yyz1.discourse-cdn.com/flex027/user_avatar/forum.biobakery.org/heleen_delbeke/32/1341_2.png) [@Heleen\_Delbeke](https://forum.biobakery.org/u/Heleen_Delbeke)\
**Post date:** [March 25, 2022, 3:42pm UTC](https://forum.biobakery.org/t/error-uploading-data-for-lefse/1089/4 "2022-03-25T15:42:08Z")

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Hello Kelsey,

I receive since 2 weeks the same error. I just old datasets that used to work and I receive the same error.

KR  
Heleen Delbeke