# Error: Unable to find the mpa\_pkl file at: mpa\_pklExiting

**URL:** https://forum.biobakery.org/t/error-unable-to-find-the-mpa-pkl-file-at-mpa-pklexiting/1568
**Category:** MetaPhlAn
**Created:** [January 19, 2021, 3:31pm UTC](https://forum.biobakery.org/t/error-unable-to-find-the-mpa-pkl-file-at-mpa-pklexiting/1568 "2021-01-19T15:31:36Z")
**Posts on this page:** 1
**Showing post:** 8

<div class="post-metadata">

### Author: ![Pearl](https://yyz1.discourse-cdn.com/flex027/user_avatar/forum.biobakery.org/pearl/32/2548_2.png) [@Pearl](https://forum.biobakery.org/u/Pearl)
#### Post date: [December 20, 2023, 5:24pm UTC](https://forum.biobakery.org/t/error-unable-to-find-the-mpa-pkl-file-at-mpa-pklexiting/1568/8 "2023-12-20T17:24:35Z")

</div>

[Marco solved this problem!](https://forum.biobakery.org/t/unable-to-find-the-mpa-pkl/503/5)

**step1: download metaphlan databases**

```auto
wget http://cmprod1.cibio.unitn.it/databases/MetaPhlAn/metaphlan_databases/mpa_v31_CHOCOPhlAn_201901.tar

```

**step2: uncompress metaphlan databases**

```auto
tar -xvf mpa_v31_CHOCOPhlAn_201901.tar

```

**step3: specific the metaphlan databases location**

```auto
humann \
--input final/ff05623391_kneaddata.fastq \
--threads 12 \
--metaphlan-options "--bowtie2db /16SrRNA/metaphlan/" \
--nucleotide-database 16SrRNA/humann/chocophlan/ \
--protein-database 16SrRNA/humann/uniref/ \
--output Microbiota/humann/

```

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_[View the full topic](https://forum.biobakery.org/t/error-unable-to-find-the-mpa-pkl-file-at-mpa-pklexiting/1568)._