# Error in MetaPhlAn installation **URL:** \ **Category:** MetaPhlAn\ **Created:** [August 14, 2023, 1:33pm UTC](https://forum.biobakery.org/t/error-in-metaphlan-installation/5769 "2023-08-14T13:33:46Z")\ **Posts on this page:** 9\ **Page:** 1
**Author:** ![mallamuneer](https://yyz1.discourse-cdn.com/flex027/user_avatar/forum.biobakery.org/mallamuneer/32/2141_2.png) [@mallamuneer](https://forum.biobakery.org/u/mallamuneer)\ **Post date:** [August 14, 2023, 1:33pm UTC](https://forum.biobakery.org/t/error-in-metaphlan-installation/5769/1 "2023-08-14T13:33:46Z")
Hi all I am trying to install MetaPhlAn and I am getting this error conda create --name mpa -c bioconda python=3.7 metaphlan Collecting package metadata (current\_repodata.json): done Solving environment: failed with repodata from current\_repodata.json, will retry with next repodata source. Collecting package metadata (repodata.json): done Solving environment: - Found conflicts! Looking for incompatible packages. This can take several minutes. Press CTRL-C to abort. failed UnsatisfiableError: The following specifications were found to be incompatible with each other: Output in format: Requested package → Available versions Package python conflicts for: python=3.7 metaphlan → python[version=‘2.7._|\>=3|\>=3.7’] metaphlan → biom-format → python[version='3.4._|3.5._|3.6._|\>=2.7,\<2.8.0a0|\>=3.5,\<3.6.0a0|\>=3.6,\<3.7.0a0|\>=3.11,\<3.12.0a0|\>=3.10,\<3.11.0a0|\>=3.9,\<3.10.0a0|\>=3.8,\<3.9.0a0|\>=3.7,\<3.8.0a0|\>=2.7,\<3|\>=3.7.1,\<3.8.0a0|\<3.0.0|\>=3.6’]The following specifications were found to be incompatible with your system: - feature:/linux-64::\_\_glibc==2.27=0 - feature:|@/linux-64::\_\_glibc==2.27=0 Your installed version is: 2.27 However, when I checked the Python --version it shows python 3.8.13 Kindly suggest ---
**Author:** ![fanhuan](https://yyz1.discourse-cdn.com/flex027/user_avatar/forum.biobakery.org/fanhuan/32/451_2.png) [@fanhuan](https://forum.biobakery.org/u/fanhuan)\ **Post date:** [August 18, 2023, 6:03am UTC](https://forum.biobakery.org/t/error-in-metaphlan-installation/5769/2 "2023-08-18T06:03:18Z")
I’m having a similar issue about glibc (pythong version was fine). $ conda create --name biobakery3 python=3.7 $ conda activate biobakery3 (biobakery3)$ conda install -c bioconda metaphlan=4.0.6 Collecting package metadata (current\_repodata.json): done Solving environment: failed with initial frozen solve. Retrying with flexible solve. Solving environment: failed with repodata from current\_repodata.json, will retry with next repodata source. Collecting package metadata (repodata.json): done Solving environment: failed with initial frozen solve. Retrying with flexible solve. Solving environment: | Found conflicts! Looking for incompatible packages. This can take several minutes. Press CTRL-C to abort. failed UnsatisfiableError: The following specifications were found to be incompatible with each other: Output in format: Requested package → Available versionsThe following specifications were found to be incompatible with your system: - feature:/linux-64::\_\_glibc==2.31=0 - feature:|@/linux-64::\_\_glibc==2.31=0 Your installed version is: 2.31 ---
**Author:** ![mallamuneer](https://yyz1.discourse-cdn.com/flex027/user_avatar/forum.biobakery.org/mallamuneer/32/2141_2.png) [@mallamuneer](https://forum.biobakery.org/u/mallamuneer)\ **Post date:** [August 18, 2023, 9:14am UTC](https://forum.biobakery.org/t/error-in-metaphlan-installation/5769/3 "2023-08-18T09:14:56Z")
Hi @fanhuan Dud you got the error fixed. I am trying an alternative way by installing biobakery\_workflows But that again gives me error. Meanwhile I am waiting for the reply from the group members and experts in the groups. ---
**Author:** ![Makio](https://yyz1.discourse-cdn.com/flex027/user_avatar/forum.biobakery.org/makio/32/2365_2.png) [@Makio](https://forum.biobakery.org/u/Makio)\ **Post date:** [August 24, 2023, 2:08pm UTC](https://forum.biobakery.org/t/error-in-metaphlan-installation/5769/4 "2023-08-24T14:08:06Z")
How about trying to use `-c conda-forge` flag as [suggested](https://github.com/biobakery/MetaPhlAn/wiki/MetaPhlAn-4)? ```auto $ conda create --name mpa -c conda-forge -c bioconda python=3.7 metaphlan ``` ---
**Author:** ![mallamuneer](https://yyz1.discourse-cdn.com/flex027/user_avatar/forum.biobakery.org/mallamuneer/32/2141_2.png) [@mallamuneer](https://forum.biobakery.org/u/mallamuneer)\ **Post date:** [August 25, 2023, 6:44pm UTC](https://forum.biobakery.org/t/error-in-metaphlan-installation/5769/5 "2023-08-25T18:44:27Z")
Hi @Makio Thank you for your reply. No I didn’t, I was trying other way by installing biobakery\_workflows. But got error metaphlan --install --index mpa\_vJan21\_CHOCOPhlAnSGB\_202103 --bowtie2db bowtie2 Downloading MetaPhlAn database Please note due to the size this might take a few minutes \Downloading and uncompressing indexes Downloading [http://cmprod1.cibio.unitn.it/biobakery4/metaphlan\_databases/bowtie2\_indexes/mpa\_vJan21\_CHOCOPhlAnSGB\_202103\_bt2.tar](http://cmprod1.cibio.unitn.it/biobakery4/metaphlan_databases/bowtie2_indexes/mpa_vJan21_CHOCOPhlAnSGB_202103_bt2.tar) Downloading file of size: 19909.64 MB 14714.07 MB 73.90 % 1.81 MB/sec 47 min 45 sec Warning: Unable to download [http://cmprod1.cibio.unitn.it/biobakery4/metaphlan\_databases/bowtie2\_indexes/mpa\_vJan21\_CHOCOPhlAnSGB\_202103\_bt2.tar](http://cmprod1.cibio.unitn.it/biobakery4/metaphlan_databases/bowtie2_indexes/mpa_vJan21_CHOCOPhlAnSGB_202103_bt2.tar) Downloading [http://cmprod1.cibio.unitn.it/biobakery4/metaphlan\_databases/bowtie2\_indexes/mpa\_vJan21\_CHOCOPhlAnSGB\_202103\_bt2.md5](http://cmprod1.cibio.unitn.it/biobakery4/metaphlan_databases/bowtie2_indexes/mpa_vJan21_CHOCOPhlAnSGB_202103_bt2.md5) Warning: Unable to download [http://cmprod1.cibio.unitn.it/biobakery4/metaphlan\_databases/bowtie2\_indexes/mpa\_vJan21\_CHOCOPhlAnSGB\_202103\_bt2.md5](http://cmprod1.cibio.unitn.it/biobakery4/metaphlan_databases/bowtie2_indexes/mpa_vJan21_CHOCOPhlAnSGB_202103_bt2.md5) File “bowtie2/mpa\_vJan21\_CHOCOPhlAnSGB\_202103\_bt2.md5” not found! MD5 checksums not found, something went wrong! Please help out ---
**Author:** ![mallamuneer](https://yyz1.discourse-cdn.com/flex027/user_avatar/forum.biobakery.org/mallamuneer/32/2141_2.png) [@mallamuneer](https://forum.biobakery.org/u/mallamuneer)\ **Post date:** [August 25, 2023, 6:45pm UTC](https://forum.biobakery.org/t/error-in-metaphlan-installation/5769/6 "2023-08-25T18:45:47Z")
Hi @Makio Again, then I tried to download the database manually, but now want to know how to proceed further thanks ---
**Author:** ![Makio](https://yyz1.discourse-cdn.com/flex027/user_avatar/forum.biobakery.org/makio/32/2365_2.png) [@Makio](https://forum.biobakery.org/u/Makio)\ **Post date:** [August 25, 2023, 7:32pm UTC](https://forum.biobakery.org/t/error-in-metaphlan-installation/5769/7 "2023-08-25T19:32:24Z")
@mallamuneer you might follow the [tutorial](https://github.com/biobakery/biobakery/wiki/metaphlan4) to test the environment. I think it is good for you to download the vJan21 instead of vOct22 as I see another problem with vOct22 - [Cannot reproduce results of MetaPhlAn 4.0 tutorial - #4 by Makio](https://forum.biobakery.org/t/cannot-reproduce-results-of-metaphlan-4-0-tutorial/5809/4) ---
**Author:** ![mallamuneer](https://yyz1.discourse-cdn.com/flex027/user_avatar/forum.biobakery.org/mallamuneer/32/2141_2.png) [@mallamuneer](https://forum.biobakery.org/u/mallamuneer)\ **Post date:** [August 25, 2023, 8:15pm UTC](https://forum.biobakery.org/t/error-in-metaphlan-installation/5769/8 "2023-08-25T20:15:00Z")
Hi@ Makio thank you so much for your prompt response **First : Do you mean after manual installation, I should run the example dataset to test the environment** **Second: please share the link for vOct22** Regards ---
**Author:** ![Makio](https://yyz1.discourse-cdn.com/flex027/user_avatar/forum.biobakery.org/makio/32/2365_2.png) [@Makio](https://forum.biobakery.org/u/Makio)\ **Post date:** [August 28, 2023, 12:56pm UTC](https://forum.biobakery.org/t/error-in-metaphlan-installation/5769/9 "2023-08-28T12:56:34Z")
Hi @mallamuneer Yes, I would recommend running the example and ensuring you can reproduce the same results. vOct22 is at the official FTP site [Index of /biobakery4/metaphlan\_databases](http://cmprod1.cibio.unitn.it/biobakery4/metaphlan_databases/). Makio